Hongwei Gai is a full professor of analytical chemistry at the Jiangsu Normal University, China. His current research interests are focused on single molecules/nanoparticles optical imaging, digital immunoassay, and their applications in medical laboratory tests.Qingquan Zhang is Professor of Analytical Chemistry at Jiangsu Normal University, China. His current research activities include the development and application of digital immunoassay techniques, and droplet microfluidics.Xiaojun Liu is Professor at Jiangsu Normal University, China. Her current research focuses on the development and application of new digital immunoassays for biomarker detection.Chenghua Zong is an associate professor at Jiangsu Normal University, China. Her current research activities include the development and application of new analytical methods for biosensing and analyzing.

• 1. Introduction to Immunoassays 2. Principles of Digital Immunoassays 3. Discretization in Digital Immunoassays 4. Heterogeneous Digital Immunoassays 5. Homogeneous Digital Immunoassays 6. Applications of Digital Immunoassays 7. Outlook of Digital Immunoassays

Hongwei Gai is a full professor of analytical chemistry at the Jiangsu Normal University, China. His current research interests are focused on single molecules/nanoparticles optical imaging, digital immunoassay, and their applications in medical laboratory tests.Qingquan Zhang is Professor of Analytical Chemistry at Jiangsu Normal University, China. His current research activities include the development and application of digital immunoassay techniques, and droplet microfluidics.Xiaojun Liu is Professor at Jiangsu Normal University, China. Her current research focuses on the development and application of new digital immunoassays for biomarker detection.Chenghua Zong is an associate professor at Jiangsu Normal University, China. Her current research activities include the development and application of new analytical methods for biosensing and analyzing.

• 1. Introduction to Immunoassays 2. Principles of Digital Immunoassays 3. Discretization in Digital Immunoassays 4. Heterogeneous Digital Immunoassays 5. Homogeneous Digital Immunoassays 6. Applications of Digital Immunoassays 7. Outlook of Digital Immunoassays

Hongwei Gai is a full professor of analytical chemistry at the Jiangsu Normal University, China. His current research interests are focused on single molecules/nanoparticles optical imaging, digital immunoassay, and their applications in medical laboratory tests.Qingquan Zhang is Professor of Analytical Chemistry at Jiangsu Normal University, China. His current research activities include the development and application of digital immunoassay techniques, and droplet microfluidics.Xiaojun Liu is Professor at Jiangsu Normal University, China. Her current research focuses on the development and application of new digital immunoassays for biomarker detection.Chenghua Zong is an associate professor at Jiangsu Normal University, China. Her current research activities include the development and application of new analytical methods for biosensing and analyzing.

• 1. Introduction to Immunoassays 2. Principles of Digital Immunoassays 3. Discretization in Digital Immunoassays 4. Heterogeneous Digital Immunoassays 5. Homogeneous Digital Immunoassays 6. Applications of Digital Immunoassays 7. Outlook of Digital Immunoassays