Inositol Phosphates

Methods and Protocols

AvGregory J. Miller

Häftad, Engelska, 2020

Del i serien Methods in Molecular Biology

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Beskrivning

Chapters focus on methods to study enzymes that modify inositol signals, chemical biology of inositol phosphates, and methods to synthesize and purify inositol phosphates and their analogs.

Produktinformation

Utgivningsdatum:2020-11-27
Mått:178 x 254 x 11 mm
Vikt:380 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:185
Förlag:Springer-Verlag New York Inc.
ISBN:9781071601693

Utforska kategorier

Biokemi inom Naturvetenskap och teknik
Cellbiologi inom Naturvetenskap och teknik

Innehållsförteckning

A Short Historical Perspective of Methods in Inositol Phosphate Research.- Quantitation of Inositol Phosphates by HPLC-ESI-MS.- Isolation of Inositol Hexakisphosphate from Soils by Alkaline Extraction and Hypobromite Oxidation.- Detection of Inositol Phosphates by Split PH Domains.- Dictyostelium discoideum as a Model to Study Inositol Pyrophosphate and Inorganic Polyphosphate.- Characterising Enzymes of the Diphosphoinositol Polyphosphate Phosphohydrolase (DIPP) Family.- Metabolic Labeling of Inositol Phosphates and Phosphatidylinositols in Yeast and Mammalian Cells.- Back-pyrophosphorylation Assay to Detect in vivo InsP7-dependent Protein Pyrophosphorylation in Mammalian Cells.- Analyses of Ligand Binding to IP3 receptors using Fluorescence Polarization.- Investigating the InsP3 Receptor in Living Cells by Caged InsP3.- Optical Measurement of Synaptic Vesicle Fusion and its Inhibition by Inositol Pyrophosphate in Primary Cultured Hippocampal Neurons.- Competitive Fluorescent Ligand Assay for Inositol 1,4,5-Trisphosphate.- Studying the Effects of Inositol Pyrophosphates in an In Vitro Vesicle-vesicle Fusion Assay.- Engineering Protease Resistant and Highly Active Phytases.- Direct Cloning Method for Expression of Recombinant Proteins with an Inositol Hexakisphosphate Inducible Self-cleaving Tag.- A Short Historical Perspective of Methods in Inositol Phosphate Research.