Inositol Phosphates

Methods and Protocols

AvGregory J. Miller

Häftad, Engelska, 2020

Del i serien Methods in Molecular Biology

1 179 kr

Beställningsvara. Skickas inom 10-15 vardagar. Fri frakt över 249 kr.

Beskrivning

Chapters focus on methods to study enzymes that modify inositol signals, chemical biology of inositol phosphates, and methods to synthesize and purify inositol phosphates and their analogs.

Produktinformation

Utgivningsdatum:2020-11-27
Mått:178 x 254 x 11 mm
Vikt:380 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:185
Förlag:Springer-Verlag New York Inc.
ISBN:9781071601693

Utforska kategorier

Biokemi inom Naturvetenskap och teknik
Cellbiologi inom Naturvetenskap och teknik

Innehållsförteckning

A Short Historical Perspective of Methods in Inositol Phosphate Research.- Quantitation of Inositol Phosphates by HPLC-ESI-MS.- Isolation of Inositol Hexakisphosphate from Soils by Alkaline Extraction and Hypobromite Oxidation.- Detection of Inositol Phosphates by Split PH Domains.- Dictyostelium discoideum as a Model to Study Inositol Pyrophosphate and Inorganic Polyphosphate.- Characterising Enzymes of the Diphosphoinositol Polyphosphate Phosphohydrolase (DIPP) Family.- Metabolic Labeling of Inositol Phosphates and Phosphatidylinositols in Yeast and Mammalian Cells.- Back-pyrophosphorylation Assay to Detect in vivo InsP7-dependent Protein Pyrophosphorylation in Mammalian Cells.- Analyses of Ligand Binding to IP3 receptors using Fluorescence Polarization.- Investigating the InsP3 Receptor in Living Cells by Caged InsP3.- Optical Measurement of Synaptic Vesicle Fusion and its Inhibition by Inositol Pyrophosphate in Primary Cultured Hippocampal Neurons.- Competitive Fluorescent Ligand Assay for Inositol 1,4,5-Trisphosphate.- Studying the Effects of Inositol Pyrophosphates in an In Vitro Vesicle-vesicle Fusion Assay.- Engineering Protease Resistant and Highly Active Phytases.- Direct Cloning Method for Expression of Recombinant Proteins with an Inositol Hexakisphosphate Inducible Self-cleaving Tag.- A Short Historical Perspective of Methods in Inositol Phosphate Research.