Animal Cell Biotechnology

Methods and Protocols

AvRalf Pörtner

Häftad, Engelska, 2021

Del i serien Methods in Molecular Biology

1 261 kr

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Beskrivning

Animal Cell Biotechnology: Methods and Protocols, Fourth Edition constitutes a comprehensive manual of state-of-the-art techniques for setting up mammalian cell lines and media for development of biopharmaceuticals, and optimizing critical parameters for cell culture considering the whole cascade from the lab to the final production.

Produktinformation

Utgivningsdatum:2021-01-17
Mått:178 x 254 x 23 mm
Vikt:779 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:399
Upplaga:4
Förlag:Springer-Verlag New York Inc.
ISBN:9781071601938

Utforska kategorier

Biokemisk teknik inom Naturvetenskap och teknik
Kemi inom Naturvetenskap och teknik

Innehållsförteckning

Near-Physiological Cell-Cycle Synchronization with Countercurrent Centrifugal Elutriation.- Turning Cells into Reagents: The Preparation of Assay Ready Cells for Routine Use in Bioassays.- Screening of Media Supplements for High-Performance Perfusion Cultures by Design of Experiment.- ambr® 15 Microbioreactors for CHO Cell Cultivation.- Using a Parallel Micro-Cultivation System (Micro-Matrix) as a Process Development Tool for Cell Culture Applications.- HEK293 Cell-Based Bioprocess Development at Bench Scale by Means of On-Line Monitoring in Shake Flasks (RAMOS and SFR).- Orbitally Shaken Single-Use Bioreactor for Animal Cell Cultivation: Fed-Batch and Perfusion Mode.- Development of Mammalian Cell Perfusion Cultures at Lab Scale: From Orbitally Shaken Tubes to Benchtop Bioreactors.- Perfusion Control for High Cell Density Cultivation and Viral Vaccine Production.- How to Produce mAbs in a Cube-Shaped Stirred Single-Use Bioreactor at 200 L Scale.- Generic Workflow for the Setup of Mechanistic Process Models.- Estimation of Process Model Parameters.- Efficient Optimization of Process Strategies with Model-Assisted Design of Experiments.- Design, Optimization, and Adaptive Control of Cell Culture Seed Trains.- High-Throughput Quantification and Glycosylation Analysis of Antibodies using Bead-Based Assays.- Surface Plasmon Resonance-Based Method for Rapid Product Sialylation Assessment in Cell Culture.- Analysis of Product Quality of Complex Polymeric IgM Produced by CHO Cells.- Raman Trapping Microscopy for Non-Invasive Analysis of Biological Samples.- An Optical Biosensor for Continuous Glucose Monitoring in Animal Cell Cultures.- Turbidimetry and Dielectric Spectroscopy as Process Analytical Technologies for Mammalian and Insect Cell Cultures.- Continuous Chromatography Purification of Virus-Based Biopharmaceuticals: A Shortcut Design Method.- Single Step Purification of Glycogen Synthase Kinase Isoforms from Small Scale Transient Expression in HEK293 Cells with a Calcium-Dependent Fragment Complementation System.