Animal Cell Biotechnology

Methods and Protocols

AvRalf Pörtner

Inbunden, Engelska, 2013

Del i serien Methods in Molecular Biology

1 589 kr

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Beskrivning

Animal Cell Biotechnology: Methods and Protocols, Third Edition constitutes a comprehensive manual of state-of-the-art and new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture from lab to final production.

Produktinformation

Utgivningsdatum:2013-12-04
Mått:178 x 254 x 33 mm
Vikt:1 098 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:498
Upplaga:3
Förlag:Humana Press Inc.
ISBN:9781627037327

Utforska kategorier

Innehållsförteckning

High-Throughput Synchronization of Mammalian Cell Cultures by Spiral Microfluidic Sorter.- Insect Cell Line Development Using Flp-Mediated Cassette Exchange Technology.- Scalable Transient Gene Expression in Adherent Mammalian Cells Using Polyethylenimine.- Scalable Transient Protein Expression.- DNA Profiling and Characterisation of Animal Cell Lines.- Tools for High-Throughput Process and Medium Optimization.- Designing Media for Animal Cell Culture – CHO Cells, The Industrial Standard.- Feed Optimization in Fed-Batch Culture.- Screening and Optimization of Chemically-Defined Media and Feeds with Integrated and Statistical Approaches.- Evaluation of Solid and Porous Microcarriers for Cell Growth and Production of Recombinant Proteins.- Microbioreactors and Scale-Down Models: Growth of CHO Cells Using the Pall Micro24 MicroReactor System.- Monitoring Cell Growth, Viability and Apoptosis.- Metabolic Flux Estimation in Mammalian Cell Cultures.- Quenching Methods for the Analysis of Intracellular Metabolites.- NMR Methods for Metabolomics of Mammalian Cell Culture Bioreactors.- 1H-NMR Protocol for Exometabolome Analysis of Cultured Mammalian Cells.-Microfluidic Perfusion Culture.- Disposable Bioreactors for Inoculum Production and Protein Expression.- Configuration of Bioreactors.- Process Analytical Technology (PAT) in Insect and Mammalian Cell Culture Processes – Dielectric Spectroscopy and Focused Beam Reflectance Measurement (FBRM).- Cell Assessment by Atline Microscopy.- Seed Train Optimization for Cell Culture.- Vaccine Production - Upstream Processing with Adherent or Suspension Cell Lines.- Efficient Processes for Protein Expression Using Recombinant Baculovirus Particles.- Chromatographic Techniques in the Downstream Processing of Proteins in Biotechnology.- Virus Separation Using Membranes.