RNA Interference and CRISPR Technologies

Technical Advances and New Therapeutic Opportunities

AvMouldy Sioud

Häftad, Engelska, 2021

Del i serien Methods in Molecular Biology

1 748 kr

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Beskrivning

This volume explores the uses of RNAi and CRISPR interferences as a general method for inhibiting gene expression, with focus on their biological functions, design, chemical modifications, delivery, and preclinical/clinical applications.

Produktinformation

Utgivningsdatum:2021-02-01
Mått:178 x 254 x 28 mm
Vikt:938 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:486
Förlag:Springer-Verlag New York Inc.
ISBN:9781071602928

Utforska kategorier

Biovetenskap inom Naturvetenskap och teknik

Innehållsförteckning

RNA and CRISPR Interferences: Past, Present and Future Perspectives.- Chemical Modifications in RNA Interference and CRISPR/Cas Genome Editing Reagents.- Preparation, Determination of Activity, and Biodistribution of Cholesterol-Containing Nuclease-Resistant siRNAs In Vivo.- Multifunctional Nanodelivery Platform for Maximizing Nucleic Acids Combination Therapy.- PAMAM Dendrimers as a Delivery System for Small Interfering RNA.- Delivery of Functional Small RNAs via Extracellular Vesicles In Vitro and In Vivo.- Optimized siRNA Delivery into Primary Immune Cells using Electroporation.- Synthesis and Evaluation of Caged siRNAs with Single cRGD Modification for Photoregulating RNA Interference.- Exploring 5’-Biotinylation of the Sense Strand to Improve siRNA Specificity and Potency.- In Vivo Delivery of Cassettes Encoding Anti-HBV Primary MicroRNAs using an Ancestral Adeno-Associated Viral Vector.- Generating DNA Expression Cassettes Encoding Multimeric Artificial MicroRNA Precursors.- MiR-302-Mediated Somatic Cell Reprogramming and Method for Generating Tumor-Free iPS Cells Using miR-302.- Urinary MicroRNAs as Emerging Class of Noninvasive Biomarkers.- Improving Dendritic Cell Cancer Vaccine Potency using RNA Interference.- Unleashing the Therapeutic Potential of Dendritic and T Cell Therapies using RNA Interference.- Harnessing the Antiviral-Type Responses Induced by Immunostimulatory siRNAs for Cancer Immunotherapy.- Cancer Immunotherapy: Targeting Tumor-Associated Macrophages by Gene Silencing.- Use of RNA Interference with TCR Transfer Enhance Safety and Efficiency.- CRISPR/Cas9 Guide RNA Design Rules for Predicting Activity.- CRISPR/Cas9 Genome Editing in Human Cell Lines with DONOR Vector Made by Gibson Assembly.- Genome Editing in Zebrafish using High-Fidelity Cas9 Nucleases: Choosing the Right Nuclease for the Task.- Next Generation of Adoptive T Cell Therapy using CRISPR/Cas9 Technology: Universal or Boosted?.- Engineering T-Cells using CRISPR/Cas9 for Cancer Therapy.- CRISPR/Cas9 Mediated Genome Engineering of Primary Human B Cells.- Gene Editing in B-Lymphoma Cell Lines using CRISPR/Cas9 Technology.- Gene Knockout in Hematopoietic Stem and Progenitor Cells followed by Granulocytic Differentiation.- CRISPR/Cas9 Genome Editing of Human Induced Pluripotent Stem Cells followed by Granulocytic Differentiation.