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This detailed new edition delves into recently developed protocols for those working on cell nucleus research, including some of the less well-explored areas of study.
Live-Cell Imaging and Analysis of Nuclear Body Mobility.- Laser Targeted Oligo Ligation (LTOL) to Identify DNA Sequences in the Vicinity of a Single Subnuclear Structure in a Single Cell.- Visualizing Chromatin Modifications in Isolated Nuclei.- Dual-Color Metal-Induced Energy Transfer (MIET) Imaging for Three-Dimensional Reconstruction of Nuclear Envelope Architecture.- Studying Proton Gradients Across the Nuclear Envelope.- COMBinatorial Oligonucleotide FISH (COMBO-FISH) with Uniquely Binding Repetitive DNA Probes.- Genome-Wide Mapping of UV-Induced DNA Damage with CPD-Seq.- AP-Seq: A Method to Measure Apurinic Sites and Small Base Adducts Genome-Wide.- Locus-Specific Chromatin Proteome Revealed by Mass Spectrometry-Based CasID.- Methyl Adenine Identification (MadID): High-Resolution Detection of Protein-DNA Interactions.- Optimized Detection of Protein-Protein and Protein-DNA Interactions, with Particular Application to Plant Telomeres.- Macromolecular Crowding Measurementswith Genetically Encoded Probes Based on Förster Resonance Energy Transfer in Living Cells.- Analysis of a Nuclear Intrinsically Disordered Proteome.- Timing of Cytosine Methylation on Newly-Synthesized RNA by Electron Microscopy.- The Nucleus of Intestinal Cells of the Bacterivore Nematode Caenorhabditis elegans as a Sensitive Sensor of Environmental Pollutants.