Plant Chemical Genomics

Methods and Protocols

AvGlenn R. Hicks,Chunhua Zhang

Häftad, Engelska, 2021

Del i serien Methods in Molecular Biology

1 378 kr

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Beskrivning

This second edition volume is a companion volume to the previous edition and looks at new findings on novel bioactive chemicals and cognate targets, as well as the use of synthetic small molecules and a variety of tools to understand these processes.

Produktinformation

Utgivningsdatum:2021-12-04
Mått:178 x 254 x 12 mm
Vikt:402 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:196
Upplaga:2
Förlag:Springer-Verlag New York Inc.
ISBN:9781071609569

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Innehållsförteckning

Chemical Genetics to Uncover Mechanisms Underlying Lipid-Mediated.- Signaling Events in Plants.- Method for Phenotypic Chemical Screening to Identify Cryptochrome Inhibitor.- Whole Seedling Based Chemical Genetic Screens in Arabidopsis.- Identification of Type iii Secretion Inhibitors for Plant Disease Management.- Investigation of Drug Efficacy by Screening Bioactive Chemical Effects on Plant Cell Subcellular Architecture.- In Vivo Chemical Genomics with Random Cyclized Peptides.- Interfering Peptides Targeting Protein-Protein Interactions in the Ethylene Plant Hormone Signaling Pathway as Tools to Delay Plant Senescence.- The Screening for Novel Inhibitors of Auxin-Induced Ca2+ Signaling.- Identification of ABA Receptor Agonists Using a Multiplexed High-Throughput Chemical Screening.- A Luciferase Reporter Assay to Identify Chemical Activators of ABA Signaling.- Identification of Novel Molecular Regulators Modulating Ethylene Biosynthesis Using EMS-Based Genetic Screening.- Investigation of Auxin Biosynthesis and Action Using Auxin Biosynthesis Inhibitors.- Target Profiling of an Anticancer Drug Curcumin by an In Situ Chemical Proteomics Approach.- Label-Free Target Identification and Confirmation Using Thermal Stability Shift Assays.- Drug Affinity Responsive Target Stability (DARTS) Assay to Detect Interaction Between a Purified Protein and a Small Molecule.- Using Differential Scanning Fluorimetry (DSF) to Detect Ligand Binding with Purified Protein.- Microscale Thermophoresis (MST) to Detect the Interaction between Purified Protein and Small Molecule.

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