Human Cytomegaloviruses

Methods and Protocols

AvAndrew D. Yurochko

Inbunden, Engelska, 2021

Del i serien Methods in Molecular Biology

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Beskrivning

This new edition explores and provides an update on the biology and pathogenesis of human cytomegalovirus infection.

Produktinformation

Utgivningsdatum:2021-02-09
Mått:178 x 254 x 32 mm
Vikt:1 092 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:468
Upplaga:2
Förlag:Springer-Verlag New York Inc.
ISBN:9781071611104

Utforska kategorier

Fysiologi inom Medicin
Medicinsk utrustning och medicinska tekniker inom Medicin
Biologi inom Naturvetenskap och teknik

Innehållsförteckning

Overview of Human Cytomegalovirus Pathogenesis.- Distinct Properties of Human Cytomegalovirus Strains and the Appropriate Choice of Strains for Particular Studies.- Use of Diploid Human Fibroblasts as a Model System to Culture, Grow, and Study Human Cytomegalovirus Infection.- The Use of Primary Human Cells to Analyze Human Cytomegalovirus Biology.- Human Hematopoietic Long-Term Culture (hLTC) for Human Cytomegalovirus Latency and Reactivation.- Collection and Isolation of CD14+ Primary Human Monocytes via Dual Density Centrifugation as a Model System to Study Human Cytomegalovirus Infection and Pathogenesis.- Stable and Inducible Gene Knockdown in Primary Human Fibroblasts: A Versatile Tool to Study the Role of Human Cytomegalovirus Host Cell Factors.- Construction of Human Cytomegalovirus Mutants with Markerless BAC Mutagenesis.- Methods for Studying the Function of Cytomegalovirus GPCRs.- Analysis of Cytomegalovirus Glycoprotein and Cellular Receptor Interactions.- Antibody-Independent Quantification of Cytomegalovirus Virion Protein Incorporation Using HiBiT.- Use of a Phosphoproteomic Screen to Profile Early Changes during HCMV Infection of Human Monocytes.- A Generally Applicable CRISPR/Cas9 Screening Technique to Identify Host Genes Required for Virus Infection as Applied to Human Cytomegalovirus (HCMV) Infection of Epithelial Cells.- Quantitative Electron Microscopy to Study HCMV Morphogenesis.- Detection of Cytomegalovirus Interleukin 10 (cmvIL-10) by Enzyme-Linked Immunosorbent Assay (ELISA).- Techniques for Characterizing Cytomegalovirus-Encoded miRNAs.- Development of a huBLT Mouse Model to Study HCMV Latency, Reactivation, and Immune Response.- Rodent Models of Congenital Cytomegalovirus Infection.- Recent Approaches and Strategies in the Generation of Anti-Human Cytomegalovirus Vaccines.