Ras Activity and Signaling

Methods and Protocols

AvIgnacio Rubio,Ian Prior

Inbunden, Engelska, 2021

Del i serien Methods in Molecular Biology

2 153 kr

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Beskrivning

This volume compiles new experimental approaches and concepts focusing mostly, but not solely, on ways to manipulate and regulate Ras activity and its downstream signaling output.

Produktinformation

Utgivningsdatum:2021-05-12
Mått:178 x 254 x 30 mm
Vikt:1 041 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:439
Förlag:Springer-Verlag New York Inc.
ISBN:9781071611890

Utforska kategorier

Biokemi inom Naturvetenskap och teknik

Innehållsförteckning

Ras Variant Biology and Contributions to Human Disease.- Regulation of the Small GTPase Ras and its Relevance to Human Disease.- Precise Characterization of KRAS4B Proteoforms by Combining Immunoprecipitation with Top-Down Mass Spectrometry.- Absolute Quantitation of GTPase Protein Abundance.- Validation of Isoform- and Mutation-specific RAS Antibodies.- Production and Membrane Binding of N-terminally Acetylated, C-terminally Farnesylated and Carboxymethylated KRAS4b.- Active GTPase Pulldown Protocol.- Methods to Monitor Ras Activation State.- NMR Detection Methods for Profiling RAS Nucleotide Cycling.- Ras Diffusion and Interactions with the Plasma Membrane Measured by FRAP Variations.- Understanding Ras Spatial Cycles through Reaction-Diffusion Simulations.- Super-Resolution Imaging and Spatial Analysis of RAS on Intact Plasma Membrane Sheets.- FLIM-FRET Analysis of Ras Nanoclustering and Membrane-Anchorage.- Assessment of Plasma Membrane Fatty Acid Composition and Fluidity Using Imaging Flow Cytometry.- Spatiotemporal Imaging of Small GTPase Activity using Conformational Sensors for GTPase Activity (COSGA).- Using BioID to Characterize the RAS Interactome.- Probing RAS function with Monobodies.- Detection of Endogenous RASSF1A Interacting Proteins.- Mathematical Modeling to Study KRAS Mutant-Specific Responses to Pathway Inhibition.- A Facile Method to Engineer Mutant Kras Alleles in an Isogenic Cell Background.- RASless MEFs as a Tool to Study RAS-dependent and -independent Functions.- Generation of Patient-derived Colorectal Cancer Organoids for RAS Studies.- Ras-GEF Mouse Models for the Analysis of Ras Biology and Signaling .- Studying Metabolic Abnormalities in the Costello Syndrome HRAS G12V Mouse Model: Isolation of Mouse Embryonic Fibroblasts and Their In Vitro Adipocyte Differentiation.- Dissecting Oncogenic RAS Signalling in Melanoma Development in Genetically Engineered Zebrafish Models.- Ras, Ral, and Rap1 in C. elegans.