Ras Activity and Signaling

Methods and Protocols

AvIgnacio Rubio,Ian Prior

Inbunden, Engelska, 2021

Del i serien Methods in Molecular Biology

2 136 kr

Beställningsvara. Skickas inom 10-15 vardagar. Fri frakt över 249 kr.

Beskrivning

This volume compiles new experimental approaches and concepts focusing mostly, but not solely, on ways to manipulate and regulate Ras activity and its downstream signaling output.

Produktinformation

Utgivningsdatum:2021-05-12
Mått:178 x 254 x 30 mm
Vikt:1 041 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:439
Förlag:Springer-Verlag New York Inc.
ISBN:9781071611890

Utforska kategorier

Biokemi inom Naturvetenskap och teknik

Innehållsförteckning

Ras Variant Biology and Contributions to Human Disease.- Regulation of the Small GTPase Ras and its Relevance to Human Disease.- Precise Characterization of KRAS4B Proteoforms by Combining Immunoprecipitation with Top-Down Mass Spectrometry.- Absolute Quantitation of GTPase Protein Abundance.- Validation of Isoform- and Mutation-specific RAS Antibodies.- Production and Membrane Binding of N-terminally Acetylated, C-terminally Farnesylated and Carboxymethylated KRAS4b.- Active GTPase Pulldown Protocol.- Methods to Monitor Ras Activation State.- NMR Detection Methods for Profiling RAS Nucleotide Cycling.- Ras Diffusion and Interactions with the Plasma Membrane Measured by FRAP Variations.- Understanding Ras Spatial Cycles through Reaction-Diffusion Simulations.- Super-Resolution Imaging and Spatial Analysis of RAS on Intact Plasma Membrane Sheets.- FLIM-FRET Analysis of Ras Nanoclustering and Membrane-Anchorage.- Assessment of Plasma Membrane Fatty Acid Composition and Fluidity Using Imaging Flow Cytometry.- Spatiotemporal Imaging of Small GTPase Activity using Conformational Sensors for GTPase Activity (COSGA).- Using BioID to Characterize the RAS Interactome.- Probing RAS function with Monobodies.- Detection of Endogenous RASSF1A Interacting Proteins.- Mathematical Modeling to Study KRAS Mutant-Specific Responses to Pathway Inhibition.- A Facile Method to Engineer Mutant Kras Alleles in an Isogenic Cell Background.- RASless MEFs as a Tool to Study RAS-dependent and -independent Functions.- Generation of Patient-derived Colorectal Cancer Organoids for RAS Studies.- Ras-GEF Mouse Models for the Analysis of Ras Biology and Signaling .- Studying Metabolic Abnormalities in the Costello Syndrome HRAS G12V Mouse Model: Isolation of Mouse Embryonic Fibroblasts and Their In Vitro Adipocyte Differentiation.- Dissecting Oncogenic RAS Signalling in Melanoma Development in Genetically Engineered Zebrafish Models.- Ras, Ral, and Rap1 in C. elegans.