Neuronal Cell Culture

Methods and Protocols

AvShohreh Amini,Martyn K. White

Inbunden, Engelska, 2021

Del 2311 i serien Methods in Molecular Biology

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Beskrivning

Chapters guide readers through a general overview of the neuronal culturing principles, cell line models for neural cells, the isolation and propagation of primary cultures, stem cells, transfection and transduction of neural cultures, and other more advanced techniques.

Produktinformation

Utgivningsdatum:2021-05-26
Mått:178 x 254 x 18 mm
Vikt:620 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:205
Upplaga:2
Förlag:Springer-Verlag New York Inc.
ISBN:9781071614365

Utforska kategorier

Biovetenskap inom Naturvetenskap och teknik

Innehållsförteckning

General Overview of Neuronal Cell Culture.- Considerations for the use of SH-SY5Y Neuroblastoma Cells in Neurobiology.- Cultured cell line models of neuronal differentiation: NT2, PC12, SK-N-MC.- Murine teratocarcinoma-derived neuronal cultures.- Isolation and propagation of primary human and rodent embryonic neural progenitor cells and cortical neurons.- Mouse Enteric Neuronal Cell Culture.- Preparation of Neural Stem Cells and Progenitors: Neuronal Production and Grafting Applications.- Slice culture modeling of CNS viral infection.- Neurospheres and Glial cell cultures: immunocytochemistry for cell phenotyping.- Transfection of Neuronal Cultures.- Lentiviral Transduction of Neuronal Cells.- Compartmentalized Neuronal Cultures.- Quantitative assessment of neurite outgrowth over growth promoting or inhibitory substrats.- Isolation and Culture of Dorsal Root Ganglia (DRG) from Rodents.- Isolation of Primary Human and Rodent Brain Microvascular Endothelial Cells: Culturing, Characterization, and High-Efficiency Transfection.- Fetal Brain Injury Models of Fetal Alcohol Syndrome: Examination of Neuronal Morphologic Condition Using Sholl Assay.