Bioreactors in Stem Cell Biology

Methods and Protocols

AvKursad Turksen

Inbunden, Engelska, 2022

Del 2436 i serien Methods in Molecular Biology

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Beskrivning

Given that the use of bioreactors in cell biology is becoming more commonplace as attempts are made to scale-up production of various types of cells for regenerative medicine and pharmaceutical purposes, this volume provides practical guidance for navigating research projects.

Produktinformation

Utgivningsdatum:2022-01-21
Mått:178 x 254 x 22 mm
Vikt:743 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:273
Upplaga:2
Förlag:Springer-Verlag New York Inc.
ISBN:9781071620175

Utforska kategorier

Biokemisk teknik inom Naturvetenskap och teknik
Regenerativ medicin inom Medicin
Biologi inom Naturvetenskap och teknik

Innehållsförteckning

- Benchtop Bioreactors in Mammalian Cell Culture: Overview and Guidelines. - Volumetric Mass Transfer Coefficient Measurement in a Stirred Tank Reactor. - Fabrication Protocol for Thermoplastic Microfluidic Devices: Nanoliter Volume Bioreactors for Cell Culturing. - Expansion of Human Pluripotent Stem Cells in Stirred Tank Bioreactors. - High-Efficiency Differentiation of Human Pluripotent Stem Cells to Hematopoietic Stem/Progenitor Cells in Random Positioning Machine Bioreactors. - Integrating Human-Induced Pluripotent Stem Cell Expansion Capability and Cardiomyocyte Differentiation Potential in a Microcarrier Suspension Culture. - Chemically Defined, Xeno-Free Expansion of Human Mesenchymal Stem Cells (hMSCs) on Benchtop-Scale Using a Stirred Single-Use Bioreactor. - Large-Scale Expansion of Umbilical Cord Mesenchymal Stem Cells with Microcarrier Tablets in Bioreactor. - Optimized Method to Improve Cell Activity in 3D Scaffolds Under a Dual Real-Time Dynamic Bioreactor System. - In Vitro 3D Mechanical Stimulation to Tendon-Derived Stem Cells by Bioreactor. - Microcarrier-Supported Culture of Chondrocytes in Continuously Rocked Disposable Bioreactor. - Tracheal In Vitro Reconstruction Using a Decellularized Bio-Scaffold in Combination with a Rotating Bioreactor. - Bioreactor-Based De-epithelialization of Long-Segment Tracheal Grafts. - Production of Extracellular Vesicles Using a CELLine Adherent Bioreactor Flask. - Extracellular Vesicle Collection from Human Stem Cells Grown in Suspension Bioreactors. - Bacterial Nanocellulose-Based Grafts for Cell Colonization Studies: An In Vitro Bioreactor Perfusion Model. - A Guideline to Set Up Cascaded Continuous Cultivation with E. coli Bl21 (DE3). - Applying Stirred Perfusion to 3D Tissue Equivalents to Mimic the Dynamic In Vivo Microenvironment. - Bioreactor-Based Adherent CellsHarvesting from Microcarriers with 3D Printed Inertial Microfluidics.