Non-Alcoholic Steatohepatitis

Methods and Protocols

AvDevanand Sarkar

Inbunden, Engelska, 2022

Del i serien Methods in Molecular Biology

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Beskrivning

This volume contains a comprehensive collection of laboratory protocols used by researchers to analyze varied aspects of non-alcoholic steatohepatitis (NASH).

Produktinformation

Utgivningsdatum:2022-02-26
Mått:178 x 254 x 26 mm
Vikt:910 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:361
Förlag:Springer-Verlag New York Inc.
ISBN:9781071621271

Utforska kategorier

Biologi inom Naturvetenskap och teknik
Medicinsk genetik inom Medicin

Innehållsförteckning

Histopathological Diagnosis of Nonalcoholic Steatohepatitis (NASH).- Generation of a Diet-Induced Mouse Model of Nonalcoholic Fatty Liver Disease.- Hydrodynamic Injection for Developing NASH Model.- Measurement of Hepatic Lipids.- Measurement of Fatty Acid Oxidation in Mammalian Cells.- Measurement of In Vivo VLDL and Chylomicron Secretion.- Isolation and Culture of Mouse Hepatocytes and Kupffer Cells (KC).- Mouse Bone Marrow Cell Isolation and Macrophage Differentiation.- Purification and Isolation of Hepatic Stellate Cells.- Isolation of the Stromal Vascular Fraction from Adipose Tissue and Subsequent Differentiation into White or Beige Adipocytes.- Culture of Mouse Liver Ductal Organoids.- Development of a Scalable Three-Dimensional Culture of Human Induced Pluripotent Stem Cells-Derived Liver Organoids.- Chromatin Immunoprecipitation Assay in Primary Mouse Hepatocytes and Mouse Liver.- Analysis of Liver Responsesto Non-Alcoholic Steatohepatitis by mRNA-Sequencing.- Single-Cell RNA-Sequencing in NASH.- Computational Pipeline for Next-Generation Sequencing (NGS) Studies in Genetics of NASH.- Metabolic Phenotyping in Mice with NASH using Indirect Calorimetry.- Analysis of Insulin Resistance in Nonalcoholic Steatohepatitis.- Assessment of Lipotoxic Endoplasmic Reticulum (ER) Stress in Nonalcoholic Steatohepatitis (NASH).- Ex Vivo Dual-Hit Method for Inflammasome Activation in Liver.- In Vivo Analysis of Necrosis and Ferroptosis in Nonalcoholic Steatohepatitis (NASH).- Analysis of the Sphingolipidome in NAFLD.- Bile Acid Profiling in Mouse Biofluids and Tissues.- Preparation and Characterization of a Liver Targeted, Poly(amidoamine) Based, Gene Delivery System.- Extraction of Exosomes and Exosomal miRNA from Mesenchymal Stem Cells.- Generation of Adenovirus for In Vitro and In Vivo Studies of Hepatocytes.