Glycovirology

Methods and Protocols

AvYasuo Suzuki

Häftad, Engelska, 2023

Del i serien Methods in Molecular Biology

1 224 kr

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Beskrivning

Chapters guide readers through glycan analysis, glycan distribution analysis, glycan and lectin microarray, preparation of recombinant viral domain protein, reverse genetics and receptor binding, virus-host interactions and receptor binding, and sialidase: assays and inhibitors.

Produktinformation

Utgivningsdatum:2023-10-02
Mått:178 x 254 x 21 mm
Vikt:699 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:357
Förlag:Springer-Verlag New York Inc.
ISBN:9781071626375

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Innehållsförteckning

Comprehensive cellular glycan profiling of glycoproteins and glycosphingolipids by glycoblotting and BEP methods.- High-performance liquid chromatography mapping method for the structural analysis of N-glycans at the molecular, cellular, and tissue levels.- Detection of sialic acids on the cell surface using flow cytometry.- Lectin Staining to Detect Human and Avian influenza Virus Receptors in the Airway of Nonhuman Primates.- Preparation of Glycan Arrays Using Glycopeptides Derived from Biomaterials.- Glycan profiling of viral glycoproteins with the lectin microarray.- Preparation of recombinant PA endonuclease domain protein of influenza A virus and its application for glycobiology research.- Receptor-binding specificity of influenza viruses.-Functional analysis of sulphatide in influenza A virus infection and replication.- Quantification of receptor-association, -dissociation and NA-dependent motility of influenza A particles by biolayer interferometry.- Receptor-binding assay for avian influenza viruses.- Characterization of influenza virus binding to receptors on isolated cell membranes.- Characterization of human parainfluenza virus receptor using terminal sialic acid linkage-modified cells.- Solid-phase binding assay for ganglioside-binding of human respiroviruses.- Evaluation of the glycan-binding and esterase activities of hemagglutinin-esterase-fusion glycoprotein from influenza D virus.- Roles of glycans and non-glycans on the epithelium and in the immune system in H1-H18 influenza A virus infections.- Roles of sialyl glycans in HCoV-OC43, HCoV-HKU1, MERS-CoV and SARS-CoV-2 infections.- Enzymatic substrates and fluorescence imaging of influenza virus sialidase.- Assays for determining the sialidase activity of influenza viruses and monitoring influenza virus susceptibility to neuraminidase inhibitors.- Synthesis and Neuraminidase Inhibitory Activity of Sialic Acid Analogues with Fluoro, Phosphono and Sulfo Functionalities.- Influenza A virus neuraminidase inhibitors.