Fluorescent Proteins

Methods and Protocols

AvMayank Sharma

Häftad, Engelska, 2023

Del i serien Methods in Molecular Biology

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Beskrivning

This volume brings together cutting-edge laboratory protocols to characterize the novel fluorescent proteins (FPs) and approaches based on fluorescent proteins that aim to answer some of the key cell biological questions.

Produktinformation

Utgivningsdatum:2023-09-17
Mått:178 x 254 x 19 mm
Vikt:641 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:326
Förlag:Springer-Verlag New York Inc.
ISBN:9781071626696

Utforska kategorier

Biokemi inom Naturvetenskap och teknik
Cellbiologi inom Naturvetenskap och teknik

Innehållsförteckning

Using FPbase: The Fluorescent Protein Database.- Selecting the Fluorescent Protein for In Vivo Imaging Experiments.- Advanced Fluorescence Microscopy Methods to Study Dynamics of Fluorescent Proteins In Vivo.- Directed Evolution of Fluorescent Proteins in Bacteria.- Fluorescent Proteins: Crystallization, Structural Determination, and Non-Natural Amino Acid Incorporation.- Development and Characterization of Flavin-Binding Fluorescent Proteins, Part I: Basic Characterization.- Development and Characterization of Flavin-Binding Fluorescent Proteins, Part II: Advanced Characterization.- CRISPR-Cas9-Mediated Knock-In Approach to Insert the GFP11 Tag into the Genome of a Human Cell Line.- Fluorescent Protein-Based Approaches for Sub-Cellular Protein Localization in Plants.- Growth Rates Evaluation of the Budding Yeast Saccharomyces cerevisiae Cells Carrying Endogenously Expressed Fluorescent Protein Fusions.- DNA Visualization Using Fluorescent Proteins.- Quantification ofIntracellular Citrate Concentrations with Genetically Encoded Biosensors.- Quantification of Redox-Sensitive GFP Cysteine Redox State via Gel-Based Read-Out.- Assessing In Vivo Oxygen Dynamics Using Plant N-Terminal Degrons in Saccharomyces cerevisiae.- Osmium Resistant Fluorescent Proteins and In-Resin Correlative Light-Electron Microscopy of Epon-Embedded Mammalian Cultured Cells.- Fluorescence-Detection Size-Exclusion Chromatography-Based Thermostability Assay for Membrane Proteins.- Determining Protein-Protein Interaction with GFP-Trap Beads.