MicroRNA Profiling

Methods and Protocols

AvSweta Rani

Häftad, Engelska, 2023

Del i serien Methods in Molecular Biology

1 404 kr

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Beskrivning

Chapters detail miRNA biogenesis, isolating RNA, extracellular vesicles (EVs), circulating miRNAs, analyzing miRNA and miRDeep-P2, protocols for total RNA isolation from cells, cell-derived products, isolation and characterization of exosomes, serum, plasma specimens, and software tools.

Produktinformation

Utgivningsdatum:2023-11-29
Mått:178 x 254 x 15 mm
Vikt:511 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:252
Upplaga:2
Förlag:Springer-Verlag New York Inc.
ISBN:9781071628256

Utforska kategorier

Biovetenskap inom Naturvetenskap och teknik

Innehållsförteckning

miRNA Biogenesis and Regulation of Diseases: An updated Overview.- Exosomal MicroRNA profiling.- MicroRNA expression profiling using Agilent One-Color Microarray.- A simple method for profiling and analysing microRNAs from small volume samples using a qPCR-based platform.- Exosomal microRNAs: comprehensive methods from exosome isolation to miRNA extraction and purity analysis.- Detection of MicroRNAs in Brain Slices Using In Situ Hybridization.- MicroRNA profiling of cell lines and xenografts by quantitative PCR.- Assessment of basic biological functions exerted by miRNAs.- Serum microRNAs Profiling in Age-Related Macular Degeneration.- Exosomal microRNA discovery in Age-Related Macular Degeneration.- MicroRNA profiling using a PCR based method.- Guidelines on designing microRNA sponges: from construction to stable cell line.- Evaluating single-nucleotide variants in microRNAs targeting sites and mature microRNAs in vitro cell culture by luciferase reporter gene assays.- Assessment of cell cytotoxicity in 3D biomaterial scaffolds following miRNA transfection.- Evaluation of miRNA expression in 3D in vitro scaffold-based cancer models.- Bioinformatics Analysis of MiRNA sequencing Data.- Plant microRNA identification and annotation using deep sequencing data.