Chaperones

Methods and Protocols

AvStuart K. Calderwood,Thomas L. Prince

Häftad, Engelska, 2024

Del i serien Methods in Molecular Biology

1 682 kr

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Beskrivning

This second edition volume expands on the previous edition with new discussions on the latest techniques used to study molecular chaperones and the stress response.

Produktinformation

Utgivningsdatum:2024-08-05
Mått:178 x 254 x 19 mm
Vikt:648 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:327
Upplaga:2
Förlag:Springer-Verlag New York Inc.
ISBN:9781071633441

Utforska kategorier

Biokemi inom Naturvetenskap och teknik

Innehållsförteckning

Monitoring of the Heat Shock Response with a Real-Time Luciferase Reporter.- Studying RNA Polymerase II Promoter-Proximal Pausing by In Vitro Immobilized Template and Transcription Assays.- Role of Heat Shock Factors in Stress-Induced Transcription: An Update.- A Workflow Guide to RNA-seq Analysis of Chaperone Function and Beyond.- Chromatin Immunoprecipitation (ChIP) of Heat Shock Protein 90 (Hsp90).- Transfection and Thermotolerance Methods for Analysis of miR-570 Targeting the HSP Chaperone Network.- Targeted Replacement of HSF1 Phosphorylation Sites at S303/S307 with Alanine Residues in Mice Increases Cell Proliferation and Drug Resistance.- Bimolecular Fluorescence Complementation Assay to Evaluate HSP90-Client Protein Interactions in Cells.- Complementation Assays for Co-Chaperone Function.- Optimized Microscale Protein Aggregation Suppression Assay: A Method for Evaluating the Holdase Activity of Chaperones.- Detecting Post-Translational Modifications of Hsp90 Isoforms.- Multiple Targeting of HSP Isoforms to Challenge Isoform Specificity and Compensatory Expression.- Using a Modified Proximity Ligation Protocol to Study the Interaction between Chaperones and Associated Proteins.- Use of Native-PAGE for the Identification of Epichaperomes in Cell Lines.- Molecular Chaperone Receptors: An Update.- A Novel Heat Shock Protein 70-Based Vaccine Prepared from DC-Tumor Fusion Cells: An Update.- Methods to Assess the Impact of Hsp90 Chaperone Function on Extracellular Client MMP2 Activity.- Proteome and Chaperone Analysis of Extracellular Vesicles.- A Modified Differential Centrifugation Protocol for Isolation and Quantitation of Extracellular Heat Shock Protein 90 (eHsp90).- Immunohistochemistry of Human Hsp60 in Health and Disease: Recent Advances in Immuno-Morphology and Methods for Assessing the Chaperonin in Extracellular Vesicles.- Multiplex Immunostaining Method to Distinguish HSP Isoforms in Cancer Tissue Specimens.- Large-Scale Databases and Portals on Cancer Genome to Analyze Chaperone Genes Correlated to Patient Prognosis.- Immunohistochemical, Flow Cytometric and ELISA-Based Analyses of Intracellular, Membrane-Expressed, and Extracellular Hsp70 as Cancer Biomarkers.