3D Cell Culture

Methods and Protocols

AvZuzana Sumbalova Koledova

Häftad, Engelska, 2025

Del 2764 i serien Methods in Molecular Biology

1 701 kr

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Beskrivning

Since the publication of the previous edition, the use of 3D cell and organoid cultures has become more widespread across laboratories.

Produktinformation

Utgivningsdatum:2025-03-12
Mått:178 x 254 x 19 mm
Vikt:655 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:336
Upplaga:2
Förlag:Springer-Verlag New York Inc.
ISBN:9781071636763

Utforska kategorier

Innehållsförteckning

3D Cell Culture: Techniques For and Beyond Organoid Applications.- Preparation of Corneal Tissue Matrix Bioink for 3D Bioprinting.- Decellularization of Pig Lung to Yield Three-Dimensional Scaffold for Lung Tissue Engineering.- Three-Dimensional Hanging Drop Spheroid Plates for Easy Chimeric Antigen Receptor (CAR) T Cytotoxicity Assay.- Static 3D Osteoblast Cell Culture on 3D Printed Titanium Scaffolds.- Modelling Acute Myeloid Leukaemia using StarPEG-Heparin Hydrogels.- A Decision Tree to Guide Human and Mouse Mammary Organoid Model Selection.- Fibroblast-Epithelium Co-Culture Methods using Epithelial Organoids and Cell Line-Derived Spheroids.- Differentiation of Fibroblasts to Adipocytes in 3D for a Co-Culture with Mammary Organoids and Immunohistological Analysis.- Organotypic 3D Cell Culture of the Embryonic Lacrimal Gland.- Renal Organoids from Whole Kidney Cells.- Organotypic 3D Ex Vivo Co-Culture Model of the Macro-Metastasis/Organ Parenchyma Interface.- Addressing the Mechanical Interaction between Cancer-Associated Fibroblasts and Cancer Cells by Laser Ablation.- Double-Barrel Perfusion System for Modification of Luminal Contents of Intestinal Organoids.- Mechanical Actuation of Organoids in Synthetic Microenvironments.- 3D Breast Cancer Model on Silk Fibroin-Integrated Microfluidic Chips.- Tumor-Microenvironment-on-Chip Platform for Assessing Drug Response in 3D Dynamic Culture.- Suspended 3D Bioprinting for Engineering Miniaturized In Vitro Tumor Models.- Real-Time Cell Cycle Imaging in a 3D Cell Culture Model of Melanoma, Quantitative Analysis, Optical Clearing, and Mathematical Modeling.- Quantitative Analysis of Whole-Mount Fluorescence-Stained Tumor Spheroids in Phenotypic Drug Screens.