Embryo Models In Vitro

Methods and Protocols

AvMagdalena Zernicka-Goetz,Kursad Turksen

Häftad, Engelska, 2025

Del 2767 i serien Methods in Molecular Biology

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Beskrivning

This collection explores the use of pluripotent cells to generate early embryo-like structures in vitro, with the resultant 3D embryo-like structures in vitro phenocopying many of the developmental landmarks and 3D architectures seen normally in vivo.

Produktinformation

Utgivningsdatum:2025-02-07
Mått:178 x 254 x 19 mm
Vikt:634 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:323
Förlag:Springer-Verlag New York Inc.
ISBN:9781071636886

Utforska kategorier

Cellbiologi inom Naturvetenskap och teknik
Biokemisk teknik inom Naturvetenskap och teknik
Regenerativ medicin inom Medicin

Innehållsförteckning

Generation of Human Blastoids from Naïve Pluripotent Stem Cells.- Human Pre-Gastrulation Embryo Culture in 3D Condition.- Protocol for the Generation of Human EPS-Blastoids Using a Three-Dimensional Two-Step Induction System.- Generation of Human Trophoblast Stem Cell-Dependent Placental In Vitro Models.- Workflow for Performing Genetic Manipulation in Human Trophoblast Stem Cells Using CRISPR/Cas9 Technology.- Endometrial Assembloids to Model Human Embryo Implantation In Vitro.- Stem Cell-Derived Microfluidic Amniotic Sac Embryoid (µPASE).- Generation of 3D Trophoblast Organoids from Human Naïve Pluripotent Stem Cells.- Induction of Human Extraembryonic Mesoderm Cells from Naive Pluripotent Stem Cells.- Modeling Human Paraxial Mesoderm Development with Pluripotent Stem Cells.- Generation of Stem Cell-Based Mouse Embryo-Like Structures.- Embryonic Spinal Cord Innervation in Human Trunk Organogenesis Gastruloids: Cardiac Versus Enteric Customization and Beyond.- Use of Epigenetic Cues and Mechanical Stimuli to Generate Blastocyst-Like Structures from Mammalian Skin Dermal Fibroblasts.- Directed Differentiation of Human Pluripotent Stem Cells to Cytotrophoblast and Syncytiotrophoblast.- Single-Cell mRNA-sncRNA Co-Sequencing of Preimplantation Embryos.- Evaluation of Stem-Cell Embryo Models by Integration with a Human Embryo Single-Cell Transcriptome Atlas.- Inferring Gene Regulatory Networks and Predicting the Effect of Gene Perturbations via IQCELL.- ShapeMetrics: A 3D Cell Segmentation Pipeline for Single-Cell Spatial Morphometric Analysis.- Guinea Pig Preimplantation Embryos: Generation, Collection, and Immunofluorescence.- Manual Dissociation of Mammalian Preimplantation Embryos for Single-Cell Genomics.- Whole-Mount RNA, Single Molecule RNA (smRNA), and DNA Fluorescence In Situ Hybridization (FISH) in Mammalian Embryos.