Intracellular Pathogens

Methods and Protocols

AvAneesh Thakur

Inbunden, Engelska, 2024

Del 2813 i serien Methods in Molecular Biology

2 525 kr

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Beskrivning

This detailed volume presents cutting-edge approaches to study infection, immunity, and intervention strategies against intracellular pathogens.

Produktinformation

Utgivningsdatum:2024-06-19
Mått:178 x 254 x 27 mm
Vikt:925 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:373
Upplaga:2024
Förlag:Springer-Verlag New York Inc.
ISBN:9781071638897

Utforska kategorier

Patologi inom Medicin
Cellbiologi inom Naturvetenskap och teknik
Biologi inom Naturvetenskap och teknik

Innehållsförteckning

Intracellular Pathogens: Infection, Immunity, and Intervention.- Methods for Genomic Epidemiology of Bacterial Pathogens: Example Salmonella.- Phagocytosis.- Large Scale Culture and Plasmid Preparation Procedure for Low-Yield Bacmids Containing Full-Length SARS-CoV-2 cDNAs.- Metatranscriptomic RNA-Seq Data Analysis of Virus-Infected Host Cells.- Metabolic Analysis of Intracellular Pathogenic Bacteria Using NMR.- Quantitative Proteomics of the Intracellular Bacterial Pathogen Salmonella enterica Serovar Typhimurium.- Median Tissue Culture Infectious Dose 50 (TCID50) Assay to Determine Infectivity of Cytopathic Viruses.- Applications of Quantitative PCR (qPCR) in Studies of Virus-Host Interactions.- Establishing Air-Liquid Interface (ALI) Airway Culture Models for Infectious Disease Research.- Pig Acute Phase Proteins as Non-Antibody Systemic Biomarkers of Intracellular Infections.- Quantification of Mycobacterium tuberculosis Growth in Cell-Based Infection Assays by Time-Lapse Fluorescence Microscopy.- Intravital Microscopy for Imaging and Live Cell Tracking of Alveolar Macrophages in Real Time.- Recent Advancements in Radiopharmaceuticals for Infection Imaging.- Bacteriophages Isolation and Efficacy Testing.- Production, Isolation, and Detection of Poly IC in Extracellular Vesicles from U937 Cells.- In Silico Tools for Predicting Novel Epitopes.- In Situ Characterization of Human Follicular Helper CD4 T Cells.- Identification and Characterization of Antigen-Specific T-Cells in Viral Infections.- Evaluating Cationic Nanoparticles as Carriers of Antiviral Nucleic Acids.- RNA-Based Vaccines and Therapeutics Against Intracellular Pathogens.