RNA Amplification and Analysis

Methods and Protocols

AvMekbib Astatke

Inbunden, Engelska, 2024

Del 2822 i serien Methods in Molecular Biology

2 125 kr

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Beskrivning

Chapters detail RNA isolation and enrichment, RNA amplification and detection, RNA sequencing, analysis of RNA modification, RNA delivery approaches, and analysis of RNA binding and catalytic activity.

Produktinformation

Utgivningsdatum:2024-06-23
Mått:178 x 254 x 32 mm
Vikt:1 114 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:473
Upplaga:2024
Förlag:Springer-Verlag New York Inc.
ISBN:9781071639177

Utforska kategorier

Biovetenskap inom Naturvetenskap och teknik

Innehållsförteckning

RNA Isolation from Human Stem Cell Derived Retinal Organoids.- Next generation Sequencing Application a Systematic Approach for High quality RNA Isolation from Skeletal Muscles.- Ultrasound induced Enrichment of Ultra trace miRNA Biosensing in Nanoliter Samples.- Direct Detection of SARS CoV 2 RNA in Saliva with Colorimetric RT LAMP.- NASBA Coupled to Paper Microfluidics for RNA Detection.- Single cell Imaging of mRNA by Target RNA initiated RCA.- Detection of Foodborne RNA Viruses by Reverse Transcriptase Droplet Digital PCR.- Single molecule RNA Imaging in Live Cells with an Avidity based Fluorescent Light up Aptamer biRhoBAST.- Assessing MicroRNA Profiles from Low Concentration Extracellular Vesicle RNA Utilizing NanoString nCounter Technology.- Liquid and Solid Hybridization Methods to Detect RNAs.- Visualization of Individual RNA Molecules by Proximity Ligation based Chromogenic In Situ Hybridization Assay.- Quantum Dots Based Protocols for the Detection of RNAs.- RNA Analysis Using Immunoassay Detection Format.- Fluorescent Techniques for RNA Detection in Nanoparticles.- Sequencing 10X Genomics 3’ HT assay for Gene Expression.- Single Cell Sequencing of 3’ RNA Transcripts.- Short Read RNA Seq.- RNA Seq Data Analysis.- Bi clustering for Epi transcriptomic Co functional Analysis.- RNA Folding, Mutation and Detection.- Epitranscriptomic Mass Spectrometry.- Polyvinylamine and Its Derivative as Effective Carrier for Targeted Delivery of Small RNAs.- Delivery of mRNA with histidine-lysine peptides.- Plant Virus Like Particles for RNA Delivery.- Lipid-based Nanoparticles for RNA Delivery.- In vitro Amplification and Selection of Engineered RNase P ribozyme for Gene Targeting Applications.- Rapid Ribonuclease P Kinetics Measured by Stopped-Flow Fluorescence and Fluorescence Anisotropy.- Computational Design of Allosteric Ribozymes via Genetic Algorithms.