Iron Metabolism

Methods and Protocols

AvOleh Khalimonchuk

Inbunden, Engelska, 2024

Del 2839 i serien Methods in Molecular Biology

2 423 kr

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Beskrivning

This detailed volume explores classical and cutting-edge methods optimized and validated to analyze various aspects of iron metabolism, from in vitro to multi-organ level.

Produktinformation

Utgivningsdatum:2024-07-16
Mått:178 x 254 x 22 mm
Vikt:772 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:292
Upplaga:2024
Förlag:Springer-Verlag New York Inc.
ISBN:9781071640425

Utforska kategorier

Fysiologi inom Medicin
Biokemi inom Naturvetenskap och teknik
Cellbiologi inom Naturvetenskap och teknik

Innehållsförteckning

Iron Homeostatic Regulation in Saccharomyces cerevisiae: Introduction to a Computational Modeling Method.- ICP-MS Analysis of Iron from Biological Samples.- Sample Preparation for X-Ray Fluorescence Microscopy of Iron Distribution in Biological Specimen.- Proximity Ligation Assay for the Analysis of Iron Protein Interactions in the Nucleus.- Fluorescence-Based Proteoliposome Methods to Monitor Redox-Active Transition Metal Transmembrane Translocation by Metal Transporters.- Metal Uptake by Mitochondrial Carrier Family Proteins Using Lactococcus lactis.- Methods for Cultivation of Bacteroides thetaiotaomicron and Analysis of Heme Metabolism by Mass Spectrometry and Spectroscopic Approaches.- Heme o Isolation and Analysis Using Heme o Synthase Heterologously Expressed in Escherichia coli.- Fluorometric Methods to Measure Bioavailable and Total Heme.- Recombinant Biogenesis and Analysis of Cytochrome c Species.- Ultra Performance Liquid Chromatography (UPLC) Analysis of Heme Biosynthesis Intermediates.- Radiolabeling Heme and Porphyrin with 14C-Glycine or 14C -Aminolevulinic Acid.- Heterologous Expression and Purification of Eukaryotic ALA Synthase from E. coli.- Direct Spectroscopic Ferrochelatase Assay.- Reverse Thiol Trapping Approach to Assess the Thiol Status of Metal-Binding Mitochondrial Proteins.- A Chemoproteomic Approach to Monitor Native Iron-Sulfur Cluster Binding.