Clinical Metabolomics

Methods and Protocols

AvMartin Giera,Elena Sánchez-López

Inbunden, Engelska, 2024

Del 2855 i serien Methods in Molecular Biology

2 722 kr

Beställningsvara. Skickas inom 10-15 vardagar. Fri frakt över 249 kr.

Beskrivning

This second edition volume presents new and updated protocols on comprehensive compendium of clinical metabolomics protocols covering LC-MS, GC-MS, CE-MS, and NMR-based clinical metabolomics as well as bioinformatics and study design considerations.

Produktinformation

Utgivningsdatum:2024-10-02
Mått:178 x 254 x 38 mm
Vikt:1 295 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:575
Upplaga:2
Förlag:Springer-Verlag New York Inc.
ISBN:9781071641156

Utforska kategorier

Farmakologi inom Medicin
Biologi inom Naturvetenskap och teknik

Innehållsförteckning

(Pre)Clinical Metabolomics Analysis.- Global Metabolomics using LC-MS for Clinical Applications.- Modular, scalable and customizable LC-HRMS for exposomics.- Distinguishing artifactual fatty acid dimers from FAHFAs in untargeted LC-MS pipelines.- Advances in Chiral Metabolomic Profiling and Biomarker Discovery.- Stable Isotope Tracing Experiments using LC-MS.- Quantification of Total Ketone Bodies in biological matrices using Stable Isotope-labeled Internal Standards and RP-UPLC-MS/MS.- LC-MS/MS quantification of endocannabinoids in tissues.- Prostaglandin Metabolites Analysis in Urine by LC-MS/MS.- A method for analysis of oxidized phospholipids from biological samples using mass spectrometry.- Non-enzymatic oxidized polyunsaturated fatty acid products in human plasma and urine samples by LC-QTOF-MS/MS.- Reversed-Phase Ultrahigh-Performance Liquid Chromatography – Mass Spectrometry Method for High-Throughput Lipidomic Quantitation.- High-Throughput RPLC-MS/MS Quantification of Short- and Medium-Chain Fatty Acids.- Long chain base profiling with multiple reaction monitoring mass spectrometry.- Sphingolipid analysis in clinical research.- Recommendations for accurate lipid annotation and semi-absolute quantification from LC-MS/MS datasets.- Rapid analysis of Sterols in Blood Derived Samples.- Ultrahigh-Performance Supercritical Fluid Chromatography – Mass Spectrometry in the Clinical Lipidomic Analysis.- Quantification of octadecanoids in human plasma using chiral supercritical fluid chromatography-tandem mass spectrometry.- Rapid Lipid Mediator Profiling by Convergence Chromatography-MS/MS.- Analysis of the Mammalian Lipidome by DMS Shotgun Lipidomics.- Differential mobility spectrometry-based cardiolipin analysis.- CE-MS-based clinical metabolomics of human plasma.- Quantitative blood serum IVDr NMR spectroscopy in clinical metabolomics of cancer, neurodegeneration and internal medicine.- Lipoprotein (sub)fraction analysis on the Bruker B.I. LISA platform.- NMR-based stable isotope tracing of cancer metabolism.- NMR-based analysis of cellular central energy metabolism and exchange ratesIn Situ Isotope.- Tracing at Single-Cell Resolution Using Mass Spectrometry Imaging.- Extracting knowledge from MS clinical metabolomic data: Processing and analysis strategies.- Application of a computational metabolomics workflow for the diagnosis of inborn errors of metabolism in a laboratory setting.