Elena Sánchez-López - Böcker

This second edition volume presents new and updated protocols on comprehensive compendium of clinical metabolomics protocols covering LC-MS, GC-MS, CE-MS, and NMR-based clinical metabolomics as well as bioinformatics and study design considerations.

This second edition volume presents new and updated protocols on comprehensive compendium of clinical metabolomics protocols covering LC-MS, GC-MS, CE-MS, and NMR-based clinical metabolomics as well as bioinformatics and study design considerations. Chapters explore the core of several promising initiatives evolving around personalized health care and precision medicine. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Clinical Metabolomics: Methods and Protocols, Second Edition aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.

Since their first publication in 1593, the complexity and beauty of the verses by Valencian poet Ausiàs March have constituted a challenge for his readers. In this study we present an analysis of the poet’s language using an innovative methodology in the field of literary studies: the analysis of word combinations – collocations – with the support of ICT.Based on a structurally/morphosyntactically-tagged, lemmatized corpus, we carried out an automatic extraction of the collocations in March’s work by means of a search engine optimized to work with the Old Catalan language. Thus, on the basis of the more frequent characteristic patterns of this language, we were able to obtain complete lists of collocations – in a broad sense, as defined by Sinclair in his first research studies. Subsequently, we used a control corpus in order to contextualize them and describe their usage, which allowed us to establish whether the frequent combinations found belong specifically to the author – as an intrinsic part of his style –, or to the general linguistic usage of his age – as part of established Old Catalan phraseology. Our research showed us the difficulties of studying the phraseology of old languages, as well as the productivity of the collocational analysis of an author’s language.