Rice

Methods and Protocols

AvTaiji Kawakatsu

Inbunden, Engelska, 2024

Del 2869 i serien Methods in Molecular Biology

2 328 kr

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Beskrivning

Chapters detail sample preparation, temporal and spatial analysis of RNA, protein expression, transformation and precise genome editing, scalable transcriptome analysis, epigenome analysis, and gene regulatory network analysis.

Produktinformation

Utgivningsdatum:2024-11-06
Mått:178 x 254 x 17 mm
Vikt:598 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:195
Förlag:Springer-Verlag New York Inc.
ISBN:9781071642030

Utforska kategorier

Växtböcker inom Djur och Natur
Botanik inom Naturvetenskap och teknik
Biokemisk teknik inom Naturvetenskap och teknik

Innehållsförteckning

Hydroponic culture of rice seedlings for stress response assay.- Total RNA extraction from rice vegetative tissues using magnetic beads.- Total RNA extraction from starchy rice endosperm.- RNA extraction from rice immature embryo using laser capture microdissection.- Using immunoblotting for detecting of a circadian clock component, Os−GI, in rice leaves.- Preparation and Sectioning of Paraffin-Embedded Tissue for Histology and Histochemistry.- Spatial Analysis of Gene Expression by In Situ Hybridization.- Purification of Anti-OSH1 IgG and Immunohistochemical Staining of Rice Shoot Apical Meristem.- Agrobacterium-mediated transformation and targeted mutagenesis using SpCas12f in rice.- Gene targeting via CRISPR/Cas9-mediated DNA double-strand break induction in rice.- Precise base substitution using CRISPR/Cas-mediated base editor in rice.- DeLTa-seq: High-throughput targeted RNA-seq of rice leaves without RNA purification.- Lasy-seq: a high-throughput 3′ RNA-seq method for large-scale transcriptome profiling in rice.- Profiling of 24nt phasiRNAs in rice anther by massively parallel sequencing and stem-loop reverse transcription PCR.- Expression and purification of pAG-MNase for CUT&RUN.- Chromatin immunoprecipitation from shoot apices and young panicles of rice.- In vitro Genome-wide identification of transcription factor binding sites.- High-efficient transient gene transfection for transcriptional activity assay using rice protoplasts on large-scale platform.