FOXO Transcription Factors

Methods and Protocols

AvWolfgang Link

Inbunden, Engelska, 2024

Del 2871 i serien Methods in Molecular Biology

2 422 kr

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Beskrivning

Part Four discusses different fluorescence and imaging techniques to measure FOXO translocation, and Part Five focuses on functional studies in model organisms to characterize cell death regulation and life- and healthspan.

Produktinformation

Utgivningsdatum:2024-11-21
Mått:178 x 254 x 18 mm
Vikt:611 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:202
Upplaga:2
Förlag:Springer-Verlag New York Inc.
ISBN:9781071642160

Utforska kategorier

Biokemi inom Naturvetenskap och teknik

Innehållsförteckning

FOXO Transcription Factors: A Brief Overview.- Phosphorylation of FOXO Proteins as a Key Mechanism to Regulate Their Activity.- Identification of DAF-16/FOXO Binding Partners in Caenorhabditis elegans by Immunoprecipitation and Mass Spectrometry.- Measuring FOXO Activity by Using qPCR-Based Expression Analysis of FOXO Target Genes.- Reporter Gene Assays to Measure FOXO-Specific Transcriptional Activity.- Functional Motif Discovery in FOXO1 through CRISPR/Cas9 Exon Tiling Scan.- Cardiomyocyte-Specific FoxO1 Knockout Mice as Tools to Assess Cardiac Hypertrophy and Key Experimental Considerations using Cre-loxP.- Methods to Study Structure and Dynamics of FOXO Proteins.- Fluorescence Polarization Method to Assess the FOXO Phosphopeptide Interaction with 14-3-3.- Characterization of FOXO3-14-3-3 Interaction by Isothermal Titration Calorimetry.- Identification of Small-Molecule Modulators of FOXO3 through Virtual Screening.- Measuring FOXO Nuclear Shuttling Dynamics by Fluorescence Microscopy.- Fluorescent Tagging of Endogenous FOXO for Live Imaging and Pull-Down Assays.- Use of Microfluidics for Study of FOXO3 Translocation Dynamics.- Multiplexed Dual-Color Fluorescence-Based Distinction between Nuclear Trapping and Translocation of FOXO3.- Immunocytochemistry-Based Detection of FOXO Isoforms in Human Cancer and Fibroblasts.- Cell Death Regulation by Smed-foxO in the Planarian Schmidtea mediterranea.- Characterizing the Role of daf-16/ C. elegans FOXO in Lifespan and Healthspan.