HOX Genes

Methods and Protocols

AvBony De Kumar,Ashish Shelar

Inbunden, Engelska, 2025

Del 2889 i serien Methods in Molecular Biology

2 443 kr

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Beskrivning

There are also chapters on data analysis, variant calling, gene expression analysis using SmFISH, and microscopy-based approaches to study HOX gene function.

Produktinformation

Utgivningsdatum:2025-01-03
Mått:178 x 254 x 21 mm
Vikt:712 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:258
Förlag:Springer-Verlag New York Inc.
ISBN:9781071643211

Utforska kategorier

Biovetenskap inom Naturvetenskap och teknik

Innehållsförteckning

Biology of Hox Genes: Questions and Technological Challenges.- Re-Arranging the Cis-Regulatory Modules of Hox Complex in Drosophila via FLP-FRT and CRISPR/Cas9.- Tracking Abdominal-B Expression and Function in the Fly Internal Reproductive System by Explants Imaging.- Using Bimolecular Fluorescence Complementation (BiFC) with Photoactivated Localization Microscopy (PALM) to Analyze Hox/Cofactor Interactions at the Super Resolution Scale in Drosophila Salivary Glands.- Optimized smFISH Pipeline for Studying Nascent Transcription in Mouse Embryonic Tissue Samples.- Exploring Hox Genes and Their Temporal Expression in an Embryonic Model of Freshwater Crustaceans.- Investigating the Functions of Hox Genes using Planarian Asexual Reproduction.- Studying the Role of HOX Genes in Thrombocyte Development.- How to Study Gene Expression and Gain of Function of Hoxb1 in Mouse Heart Development.- Using the Conserved Hexapeptide in HOX Proteins as an Antagonist of HOX/PBX Interactions.- Detection of Protein-Nucleic Acid Interaction by Electrophoretic Mobility Shift Assay.- Capturing Chromatin Organization by MNase-seq and ATAC-seq.- A Simple Method to Analyze Context- and Tissue-Specific Cis-Regulatory Modulations of Homeotic (HOX) Genes using ChIP.- Guidelines to Analyze ChIP-Seq Data: Journey through QC and Analysis Considerations.- A Comprehensive Bioinformatics Approach to Analysis of Variants: Variant Calling, Annotation and Prioritization.- Spatial Genomic Approaches to Investigate HOX Genes in Mouse Brain Tissues.- Patterning Functional Proteins in Ultrabithorax-Based Materials.