Plant MicroRNAs

Methods and Protocols

AvStefan de Folter

Inbunden, Engelska, 2025

Del 2900 i serien Methods in Molecular Biology

2 453 kr

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Beskrivning

This second volume is a collection of new and updated protocols to study miRNA functions in plants.

Produktinformation

Utgivningsdatum:2025-05-17
Mått:178 x 254 x 26 mm
Vikt:908 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:366
Upplaga:2
Förlag:Springer-Verlag New York Inc.
ISBN:9781071643976

Utforska kategorier

Växtböcker inom Djur och Natur
Botanik inom Naturvetenskap och teknik
Biovetenskap inom Naturvetenskap och teknik

Innehållsförteckning

The Use of Arabidopsis thaliana to Characterize the Production and Action Stages of the Plant MicroRNA Pathway.- An overview of miRNA and miRNA target analysis tools.- Prediction of miRNA targets in plants.- An overview of miRNA and miRNA target analysis tools.- Scalable Approach to Evaluate Plant microRNA Trimming and Tailing from Small RNA-Seq data.- In vitro transcription of plant miRNA for structural and processing analysis.- Cost-Effective and Sensitive Probe-Based Universal (PBU) qPCR for MicroRNA Quantification in Plants.- Northern blot analysis of plant microRNAs and other small RNAs.- Northern blot analysis of plant microRNAs and other small RNAs.- Determination of expression patterns of plant miRNAs by Transcriptional Fusion using GUS and Fluorescent reporters.- A Visual Reporter System for Analyzing Small RNA-Triggered Local and Systemic Silencing of an Endogenous Plant Gene.- Unravelling tissue specific miRNAs role in plants exploiting the GAL4/UAS system. -The Luciferase reporter on intronic microRNA-directed regulation.- A simple and efficient protocol to transform and regenerate CRISPR-Cas9-mediated genome edited tomato plants.- CRISPR-activation: boosting expression of plant MIRs.- Gene silencing in plants through exogenous application of miRNAs.- A strategy to use Artificial MicroRNAs for Gene silencing and Overcome (AmiGO).- Degradome sequencing.- Whole mount staining and immunolocalization procedures for developing ovules of Vigna unguiculata.- Bioinformatic enrichment and network analyses of miRNA-related high-throughput sequencing data.- Identifying cis-regulatory binding sites in promoter regions of miRNA genes: A step-by-step protocol.- A Detailed Protocol for the Isolation of Arabidopsis thaliana Mesophyll Protoplasts and their Applications.- A protocol for live imaging of Arabidopsis gynoecium development using confocal microscopy.