This fully updated volume explores olfactory receptor research as it advances from molecular to system levels. Beginning with chapters on the large ensemble imaging method, the book continues by examining machine learning-related olfaction studies, assays to study odorant receptor activity, olfaction-related behavior techniques, and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Olfactory Receptors: Methods and Protocols, Second Edition serves as an ideal reference for newcomers to the olfaction field as well as for experienced professionals looking to master advanced methods.