DNA Damage Detection

Methods and Protocols

AvTakamitsu A. Kato

Inbunden, Engelska, 2025

Del 2933 i serien Methods in Molecular Biology

2 127 kr

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Beskrivning

mso-bidi-language: AR-SA;">Practical and timely, DNA Damage Detection: Methods and Protocols serves as an ideal guide for readers who are unfamiliar with DNA damage as well as experts seeking protocols to adapt to their own research.

Produktinformation

Utgivningsdatum:2025-05-27
Mått:178 x 254 x 15 mm
Vikt:509 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:150
Förlag:Springer-Verlag New York Inc.
ISBN:9781071645734

Utforska kategorier

Biovetenskap inom Naturvetenskap och teknik

Innehållsförteckning

Modified Alkaline Comet Assay to Detect Oxidative DNA Damage.- Radiation-Induced DNA Strand Break Detection by Gel Electrophoresis.- Quantitative Measurement of 8-Hydroxy-2′-Deoxyguanosine in Serum/Plasma and Urine Using an Enzyme-Linked Immunosorbent Assay.- Radiation-Induced Gamma-H2AX Foci Staining and Analysis.- Non-Radiolabeled Radioresistant DNA Synthesis and S-Phase Checkpoint Analysis.- Constant Field Gel Electrophoresis Analysis for DNA Double-Strand Break and Its Repair.- Detection of Chromatid Break and Micronucleus Formation Induced by Low- and High-LET Irradiation.- Measurement of DNA Polymerase and Microhomology-Mediated End-Joining Activities by Gel Electrophoresis and smFRET Techniques.- UV-Induced DNA Damage Detection by ELISA Analysis.- G2 Premature Chromosome Condensation (G2-PCC) by Calyculin A to Analyze DNA Double-Strand Breaks and Their Repair.- Mitotic Shake-Off and Cell Cycle Synchronization.- DCFDA Assay for Oxidative Stress Measurement in Fluorometer.- HPRT Mutation Assay for Chinese Hamster Ovary Cells.- Detection of De Novo Mutations by Sequencing Reduced Representation Libraries.- Chemical-Induced DNA Damage Identification by High-Throughput Detection of DNA Double-Strand Break.- Assessing DNA Damage Through the Cell Cycle Using Flow Cytometry.- A Mass Spectrometry-Based Proteomics Workflow for Concurrent Profiling of Protein Thiol Oxidation and Phosphorylation.- Analyzing Homologous Recombination Using Antibiotic Marker Substrates in Mammalian Cells.