Molecular Virology

Methods and Protocols

AvChunfu Zheng

Inbunden, Engelska, 2025

Del i serien Methods in Molecular Biology

2 111 kr

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Beskrivning

Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Produktinformation

Utgivningsdatum:2025-06-15
Mått:178 x 254 x 29 mm
Vikt:981 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:401
Förlag:Springer-Verlag New York Inc.
ISBN:9781071646144

Utforska kategorier

Biologi inom Naturvetenskap och teknik

Innehållsförteckning

Investigation of Protein Fatty Acylation in Virus-Host Interaction.- Determination of Protein Ubiquitination after Protein Denaturation in Virus-Host Interactions.- Viral Circuit Tracing in Biomedical Research: Methods, Protocols, and Applications.- Analysis of the Virus-Host Protein-Protein Interaction via Co-Immunoprecipitation (Co-IP).- Tandem Affinity Purification of Virus-Host Protein Complexes via the Strep/FLAG (SF)-TAP Tag.- Applying 3D Genome Technology in Virology Research.- In Vitro Liquid-Liquid Phase Separation Assay for Viral Proteins.- Computational Analysis of Virus-Host Interactomes.- Application of Single-Cell RNA Sequencing in Investigating Virus-Host Interactions.- CRISPR-Mediated Viral Gene Knock-In for Studying Viral-Host Interactions.- High-Throughput Yeast Two-Hybrid Technology for Investigating Molecular Virology.- Pull-Down Techniques for Determining Virus-Host Protein Interactions.- Application of Organoids to Investigate Virus-Host Interactions.- Identification of Virus-Host Protein Interactions via Proteomic Techniques.- Engineering of Vesicular Stomatitis Virus (VSV) for Modulating Cell Death and Antitumor Immunity.- 3D Tissue Culture Model for Virology Studies.- Detection of Protein Interactions During Viral Infection by Bimolecular Fluorescence Complementation.- Engineering of the WSN Strain for Investigating Antiviral and Antitumor Immunity.- The Application of HSV-1 in Investigating Neurological Diseases.- Strategy and Application of Viral Potency Assays in Cell and Gene Therapy.- Chromatin Immunoprecipitation (ChIP)-Quantitative PCR (qPCR) Analysis of Viral Genome-Host Protein Interactions.- The Application of Base Editing Technology to Investigate Virus-Host Interactions and Antiviral Therapeutic Strategies.- Zebrafish as a Model for Studying Virus-Host Interactions.- Screening for Host Proteins with Pro- and Antiviral Activity via High-Throughput CRISPR.- Isotope Labeling Techniques for Virus-Infected Cells.- Reverse Genetics-Based Methodology for Molecular Virology Study.- RBD Amplicon Sequencing and Evolutionary Analysis of SARS-CoV-2 Variants from Wastewater Samples.- RNA Binding Protein Immunoprecipitation 1 (RIP) Assay to Investigate the Interactions Between Viral RNA and Host Proteins.- Dual-Luciferase Reporter Assay to Investigate Virus-Host Interactions.- Electrophoretic Mobility Shift Assay for Identification of the Interaction Between a Viral Protein and DNA.- Applying Phage Display Technology to Obtain Specific Peptides Blocking Host-Virus Interactions.- Single Particle Cryo-EM to Investigate Nonenveloped Virus-Like Particles.- SELEX (Systematic Evolution of Ligands by Exponential Enrichment) to Identify DNA Motifs Mirroring Chromosome Targets of the Hypothetical Transcription Factor NLRP3.- Detection of Protein Interactions and Dynamics During Viral Infection via Fluorescence Resonance Energy Transfer.