Chunfu Zheng – författare

This detailed volume explores a variety of techniques for studying antiviral innate immunity and the type I interferon (IFN-I) signaling pathway. Beginning with guidelines to identify novel proteins from the host or viruses that regulate the antiviral IFN-I signaling pathway, the book continues with common and cutting-edge approaches for identifying novel regulatory factors, posttranslational modifications, as well as click chemistry to detect protein modifications. Other chapters delve into topics such as cytosolic RNA and DNA sensors, magnetic beads, cryo-electron microscopy, zebrafish, humanized mice, and organoids as model organisms to study host-virus interactions, and epidemiological approaches. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Antiviral Innate Immunity serves as an ideal guide for researchers working in this vital area of the virology and innate immunity fields.

This detailed book presents classical and cutting-edge techniques in molecular virology research. The volume begins with guidelines for investigating viral structure, proliferation processes, and virulence factors, followed by various approaches to identify key elements and molecular signaling pathways in virus-infected host cells that regulate virus-host interactions. Additional chapters explore methods for studying autophagy, post-translational modifications of virus and host proteins, epigenetic factors in anti-viral immune responses, and viral epidemiology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Molecular Virology: Methods and Protocols serves as an ideal guide to this vitally important area of study.