Proximity-Dependent Protein Biotinylation

Methods and Protocols

AvPetra Van Damme

Inbunden, Engelska, 2025

Del 2953 i serien Methods in Molecular Biology

2 111 kr

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Beskrivning

Spanning a diverse array of biological systems, including bacteria, zebrafish, plants, and mammals, this collection showcases methods that aid in the identification of protein interactions, localization, membrane topology, trafficking, and secretion dynamics.

Produktinformation

Utgivningsdatum:2025-07-11
Mått:178 x 254 x 24 mm
Vikt:836 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:326
Förlag:Springer-Verlag New York Inc.
ISBN:9781071646939

Utforska kategorier

Biokemi inom Naturvetenskap och teknik
Biologi inom Naturvetenskap och teknik

Innehållsförteckning

Cross-Species Proximity-Dependent Protein Biotinylation: A Standardized Approach for Mapping Proxeomes.- BioID in Bacteria: Selection of a Suitable Biotin Ligase for Proxeome Mapping.- Uncovering Transient Protein Interactions in the Yersinia enterocolitica Type III Secretion System Using MiniTurbo-Mediated Proximity Labeling.- Exploring Bacterial Surface Proteome Dynamics During Infection Using Proximity Labeling.- Identification of In Vivo Protein Networks Using MiniTurbo Proximity Labeling in Bacteria.- Unraveling N-Terminal Proteoform Interactomes via Multiplexed Recombineering in Salmonella.- Probing Pairwise Protein-Protein Interactions with the Bacterial POLAR Assay.- Detection and Quantification of Biotinylated Proteins for TurboID-Based Proximity Labeling Mass Spectrometry in Arabidopsis.- Detection and Quantification of Biotinylated Sites for TurboID-Based Proximity Labeling Mass Spectrometry in Arabidopsis.- Coupling Affinity Purification with Proximity Labeling in Arabidopsis: The APEAL Approach.- Characterization of the Protein Interactome of Membrane-Bound Transcription Factors Using TurboID-Based Proximity Labeling In Planta.- Identification of Cytosol-Facing Organelle Membrane-Proximal Proteins via Proximity-Dependent Biotinylation.- BioID2-Based Proximity Labeling in Adult Zebrafish.- On-Demand Proximity Labeling Using Light-Activated BioID.- Tissue-Specific Biotinylation for Interorgan Communication.- Spatiotemporally-Resolved Profiling of Protein Movement by TransitID.- An Integrated High-Throughput Proteocistromic Framework for Mapping the Interactome and Targetome of Human Transcription Factors.- Step-by-Step Application of Proximity-Dependent Biotinylation Tools for Identification of Astrocyte and Neuron Subproteomes In Vivo Across the Whole Brain.- Antibody-Mediated Protein A-APEX2 Labeling (AMAPEX) for Proximity Proteome Exploration.- A Ready-to-Use Data Analysis Pipeline for BioID Experiments Using Data-Dependent or Data-Independent Acquisition Mass Spectrometry.