This second edition provides a comprehensive collection of classic and cutting-edge methodologies as well as bioinformatics and genome-editing approaches for cancer cells and tissues. Chapters beginning with the Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge,  Immune Mediators in Cancer: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.