This fully updated edition examines recent advances in algal biotechnology and incorporates both foundational and newly developed protocols for working with algal cultivation systems and biomass. Guiding the reader from initial cultivation to advanced biochemical analysis, the book covers the rationale for using algae in biofuel production and considerations for selecting appropriate strains and cultivation systems, protocols for quantifying key biochemical components of algal biomass, including carbohydrates, lipids, and proteins, as well as what to consider when developing an algal industry such as its environmental impact and what tools and methods can be used in the continued development of this field. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Biofuels from Algae: Methods and Protocols, Second Edition serves as an ideal guide for making algal biofuel research more accessible.