Dr. Saura C. Sahu Research Chemist, Division of Toxicology, Office of Applied Research and Safety Assessment, Center for Food Safety and Applied Nutrition, US Food and Drug Administration. Dr. Sahu is the US Editor for the Journal of Applied Toxicology and the editor of “Hepatotoxicity” (Wiley, 2007), “Toxicogenomics” (Wiley, 2008), “Nanotoxicity” (Wiley, 2009), and “Handbook of Systems Toxicology” (Wiley, 2011).

• Contributors viiPreface xiAcknowledgments xiiiDedication xvAbbreviations and Acronyms xviiPart One Nanotoxicology 11 Testing Nanotoxicity: An Update of New and Traditional Methods 3Anna G. Cattaneo, Rosalba Gornati, Giovanni Bernardini, Enrico Sabbioni, Luigi Manzo and Mario Di Gioacchino2 Considerations for In Vitro Nanotoxicity Testing 35Athena M. Keene, Simona Bancos and Katherine M. Tyner3 In Vivo Evaluation of Acute and Chronic Nanotoxicity 65Hayley Nehoff, Neha N. Parayath, Sebastien Taurin and Khaled Greish4 Genotoxicity of Silver Nanoparticles 87Yan Li and Tao Chen5 Immunotoxicology of Nanomaterials 99Courtney M. Lappas6 Neurotoxicity of Nanoparticles 111Stephen C. Bondy7 Nanoparticles and Plants: From Toxicity to Activation of Growth 121Mariya V. Khodakovskaya and Mohamed H. Lahiani8 Epigallocatechin-3-gallate (EGCG) in or on Nanoparticles: Enhanced Stability and Bioavailability of EGCG Encapsulated in Nanoparticles or Targeted Delivery of Gold Nanoparticles Coated with EGCG 131Le Zhang, Shanshan Wu, Dongxu Wang, Xiaochun Wan and Jinsong ZhangPart Two Nanomedicine 1459 Factors Affecting the Oral Bioavailability of Nanomaterials 147Georgia K. Hinkley and Stephen M. Roberts10 Nanomedicine in Cancer Treatment 161Ji-Eun Kim and Myung-Haing Cho11 Nanomedicine in Diabetes: Using Nanotechnology in Prevention and Management of Diabetes Mellitus 189Radoslav Savić and Dusica Maysinger12 Inhalation Pathway as a Promising Portal of Entry: What Has to Be Considered in Designing New Nanomaterials for Biomedical Application? 205Loretta Müller, Andrea D. Lehmann, Blair D. Johnston, Fabian Blank, Peter Wick, Alke Fink and Barbara Rothen-Rutishauser13 Nanomedicine for the Brain and the Eye: Disease Management in Poorly Accessible Compartments of the Body 223Anna G. Cattaneo, Rosalba Gornati, Giovanni Bernardini, Enrico Sabbioni, Luigi Manzo and Mario Di GioacchinoPart Three Stem Cell Toxicology 24914 Stem Cells in Toxicity Testing 251Amy L. Inselman, Cheng Wang, Fang Liu and Deborah K. Hansen15 Human Stem-Cell-Derived Cardiomyocytes in Drug Discovery and Toxicity Testing 267Kornélia Szebényi, Adrienn Péntek, Nóra Varga, Zsuzsa Erdei, Gerg˝o  Vöfély, Tamás I. Orbán, Balázs Sarkadi and Ágota Apáti16 Pluripotent Stem Cells as Tools to Assess Developmental Toxicity: Diversity Instead of Consolidation 299Nicole R.L. Sparks and Nicole I. zur Nieden17 Impact of Various Nanosystems on Stem Cell Physiology 309Anamaria Orza18 Predictive Mechanisms in Stem Cells: An In Vitro System-Based Method for Testing Carcinogenicity 337Mireia Vilardell and Ralf Herwig19 Epigenetic Modifications and Stem Cell Toxicology: Searching for the Missing Link 347Zacharoula Konsoula and Frank A. Barile20 Epigenetic Modeling and Stem Cells in Toxicology Testing 359Sanket R. Gadhia and Frank A. Barile21 Use of Video Bioinformatics Tools in Stem Cell Toxicology 379Prue Talbot, Nicole I. zur Nieden, Sabrina Lin, Ivann Martinez, Ben Guan and Bir BhanuAuthor Index ISubject Index III

Dr. Saura C. Sahu Research Chemist, Division of Toxicology, Office of Applied Research and Safety Assessment, Center for Food Safety and Applied Nutrition, US Food and Drug Administration. Dr. Sahu is the US Editor for the Journal of Applied Toxicology and the editor of “Hepatotoxicity” (Wiley, 2007), “Toxicogenomics” (Wiley, 2008), “Nanotoxicity” (Wiley, 2009), and “Handbook of Systems Toxicology” (Wiley, 2011).

• Contributors viiPreface xiAcknowledgments xiiiDedication xvAbbreviations and Acronyms xviiPart One Nanotoxicology 11 Testing Nanotoxicity: An Update of New and Traditional Methods 3Anna G. Cattaneo, Rosalba Gornati, Giovanni Bernardini, Enrico Sabbioni, Luigi Manzo and Mario Di Gioacchino2 Considerations for In Vitro Nanotoxicity Testing 35Athena M. Keene, Simona Bancos and Katherine M. Tyner3 In Vivo Evaluation of Acute and Chronic Nanotoxicity 65Hayley Nehoff, Neha N. Parayath, Sebastien Taurin and Khaled Greish4 Genotoxicity of Silver Nanoparticles 87Yan Li and Tao Chen5 Immunotoxicology of Nanomaterials 99Courtney M. Lappas6 Neurotoxicity of Nanoparticles 111Stephen C. Bondy7 Nanoparticles and Plants: From Toxicity to Activation of Growth 121Mariya V. Khodakovskaya and Mohamed H. Lahiani8 Epigallocatechin-3-gallate (EGCG) in or on Nanoparticles: Enhanced Stability and Bioavailability of EGCG Encapsulated in Nanoparticles or Targeted Delivery of Gold Nanoparticles Coated with EGCG 131Le Zhang, Shanshan Wu, Dongxu Wang, Xiaochun Wan and Jinsong ZhangPart Two Nanomedicine 1459 Factors Affecting the Oral Bioavailability of Nanomaterials 147Georgia K. Hinkley and Stephen M. Roberts10 Nanomedicine in Cancer Treatment 161Ji-Eun Kim and Myung-Haing Cho11 Nanomedicine in Diabetes: Using Nanotechnology in Prevention and Management of Diabetes Mellitus 189Radoslav Savić and Dusica Maysinger12 Inhalation Pathway as a Promising Portal of Entry: What Has to Be Considered in Designing New Nanomaterials for Biomedical Application? 205Loretta Müller, Andrea D. Lehmann, Blair D. Johnston, Fabian Blank, Peter Wick, Alke Fink and Barbara Rothen-Rutishauser13 Nanomedicine for the Brain and the Eye: Disease Management in Poorly Accessible Compartments of the Body 223Anna G. Cattaneo, Rosalba Gornati, Giovanni Bernardini, Enrico Sabbioni, Luigi Manzo and Mario Di GioacchinoPart Three Stem Cell Toxicology 24914 Stem Cells in Toxicity Testing 251Amy L. Inselman, Cheng Wang, Fang Liu and Deborah K. Hansen15 Human Stem-Cell-Derived Cardiomyocytes in Drug Discovery and Toxicity Testing 267Kornélia Szebényi, Adrienn Péntek, Nóra Varga, Zsuzsa Erdei, Gerg˝o  Vöfély, Tamás I. Orbán, Balázs Sarkadi and Ágota Apáti16 Pluripotent Stem Cells as Tools to Assess Developmental Toxicity: Diversity Instead of Consolidation 299Nicole R.L. Sparks and Nicole I. zur Nieden17 Impact of Various Nanosystems on Stem Cell Physiology 309Anamaria Orza18 Predictive Mechanisms in Stem Cells: An In Vitro System-Based Method for Testing Carcinogenicity 337Mireia Vilardell and Ralf Herwig19 Epigenetic Modifications and Stem Cell Toxicology: Searching for the Missing Link 347Zacharoula Konsoula and Frank A. Barile20 Epigenetic Modeling and Stem Cells in Toxicology Testing 359Sanket R. Gadhia and Frank A. Barile21 Use of Video Bioinformatics Tools in Stem Cell Toxicology 379Prue Talbot, Nicole I. zur Nieden, Sabrina Lin, Ivann Martinez, Ben Guan and Bir BhanuAuthor Index ISubject Index III

Dr. Saura C. Sahu Research Chemist, Division of Toxicology, Office of Applied Research and Safety Assessment, Center for Food Safety and Applied Nutrition, US Food and Drug Administration. Dr. Sahu is the US Editor for the Journal of Applied Toxicology and the editor of “Hepatotoxicity” (Wiley, 2007), “Toxicogenomics” (Wiley, 2008), “Nanotoxicity” (Wiley, 2009), and “Handbook of Systems Toxicology” (Wiley, 2011).

• Contributors viiPreface xiAcknowledgments xiiiDedication xvAbbreviations and Acronyms xviiPart One Nanotoxicology 11 Testing Nanotoxicity: An Update of New and Traditional Methods 3Anna G. Cattaneo, Rosalba Gornati, Giovanni Bernardini, Enrico Sabbioni, Luigi Manzo and Mario Di Gioacchino2 Considerations for In Vitro Nanotoxicity Testing 35Athena M. Keene, Simona Bancos and Katherine M. Tyner3 In Vivo Evaluation of Acute and Chronic Nanotoxicity 65Hayley Nehoff, Neha N. Parayath, Sebastien Taurin and Khaled Greish4 Genotoxicity of Silver Nanoparticles 87Yan Li and Tao Chen5 Immunotoxicology of Nanomaterials 99Courtney M. Lappas6 Neurotoxicity of Nanoparticles 111Stephen C. Bondy7 Nanoparticles and Plants: From Toxicity to Activation of Growth 121Mariya V. Khodakovskaya and Mohamed H. Lahiani8 Epigallocatechin-3-gallate (EGCG) in or on Nanoparticles: Enhanced Stability and Bioavailability of EGCG Encapsulated in Nanoparticles or Targeted Delivery of Gold Nanoparticles Coated with EGCG 131Le Zhang, Shanshan Wu, Dongxu Wang, Xiaochun Wan and Jinsong ZhangPart Two Nanomedicine 1459 Factors Affecting the Oral Bioavailability of Nanomaterials 147Georgia K. Hinkley and Stephen M. Roberts10 Nanomedicine in Cancer Treatment 161Ji-Eun Kim and Myung-Haing Cho11 Nanomedicine in Diabetes: Using Nanotechnology in Prevention and Management of Diabetes Mellitus 189Radoslav Savić and Dusica Maysinger12 Inhalation Pathway as a Promising Portal of Entry: What Has to Be Considered in Designing New Nanomaterials for Biomedical Application? 205Loretta Müller, Andrea D. Lehmann, Blair D. Johnston, Fabian Blank, Peter Wick, Alke Fink and Barbara Rothen-Rutishauser13 Nanomedicine for the Brain and the Eye: Disease Management in Poorly Accessible Compartments of the Body 223Anna G. Cattaneo, Rosalba Gornati, Giovanni Bernardini, Enrico Sabbioni, Luigi Manzo and Mario Di GioacchinoPart Three Stem Cell Toxicology 24914 Stem Cells in Toxicity Testing 251Amy L. Inselman, Cheng Wang, Fang Liu and Deborah K. Hansen15 Human Stem-Cell-Derived Cardiomyocytes in Drug Discovery and Toxicity Testing 267Kornélia Szebényi, Adrienn Péntek, Nóra Varga, Zsuzsa Erdei, Gerg˝o  Vöfély, Tamás I. Orbán, Balázs Sarkadi and Ágota Apáti16 Pluripotent Stem Cells as Tools to Assess Developmental Toxicity: Diversity Instead of Consolidation 299Nicole R.L. Sparks and Nicole I. zur Nieden17 Impact of Various Nanosystems on Stem Cell Physiology 309Anamaria Orza18 Predictive Mechanisms in Stem Cells: An In Vitro System-Based Method for Testing Carcinogenicity 337Mireia Vilardell and Ralf Herwig19 Epigenetic Modifications and Stem Cell Toxicology: Searching for the Missing Link 347Zacharoula Konsoula and Frank A. Barile20 Epigenetic Modeling and Stem Cells in Toxicology Testing 359Sanket R. Gadhia and Frank A. Barile21 Use of Video Bioinformatics Tools in Stem Cell Toxicology 379Prue Talbot, Nicole I. zur Nieden, Sabrina Lin, Ivann Martinez, Ben Guan and Bir BhanuAuthor Index ISubject Index III