Margarida Barroso is a Professor at the Department of Molecular and Cellular Physiology, Albany Medical College (Albany, NY). Xavier Intes is a Professor in the Biomedical Engineering Department and Co-Director of the Center for Modeling, Simulation and Imaging for Medicine (CeMSIM),at Rensselaer Polytechnic Institute (Troy, U.S.).

• Section I: Overview of Imaging Methods and Instrumentation. 1. Fluorescence microscopy techniques. 2. Intra-vital microscopy, M Perro. 3. An introduction to live-cell super-resolution imaging. 4. Endoscopic Optical Coherence Tomography: Technologies and Applications. 5. Bioluminescence. 6. Macroscopic Fluorescence Imaging. 7. Optical Coherence Tomography. 8. Multiscale Photoacoustic Imaging. 9. Fluorescence Lifetime: Techniques, Analysis & Applications in the Life Sciences. Section II: Imaging cellular behavior. 10. Imaging cell metabolism. 11. Intravital imaging of cancer cell migration in vivo. 12. Imaging cellular signaling in vivo using fluorescent protein biosensors. 13. Imaging cell adhesion and migration. 14. Imaging the living eye. Section III: Whole-organ and whole-organism imaging. 15. Heart imaging. 16. Visualizing hepatic immunity through the eyes of intravital microscopy. 17. Optical imaging of the mammalian oviduct in vivo. 18. Immune system imaging. 19. Brain imaging in live mice. 20. Live imaging of zebrafish. 21. Whole-body fluorescence imaging in cancer research. 22. Large-scale fluorescence imaging in neuroscience

Margarida Barroso is a Professor at the Department of Molecular and Cellular Physiology, Albany Medical College (Albany, NY). Xavier Intes is a Professor in the Biomedical Engineering Department and Co-Director of the Center for Modeling, Simulation and Imaging for Medicine (CeMSIM),at Rensselaer Polytechnic Institute (Troy, U.S.).

• Section I: Overview of Imaging Methods and Instrumentation. 1. Fluorescence microscopy techniques. 2. Intra-vital microscopy, M Perro. 3. An introduction to live-cell super-resolution imaging. 4. Endoscopic Optical Coherence Tomography: Technologies and Applications. 5. Bioluminescence. 6. Macroscopic Fluorescence Imaging. 7. Optical Coherence Tomography. 8. Multiscale Photoacoustic Imaging. 9. Fluorescence Lifetime: Techniques, Analysis & Applications in the Life Sciences. Section II: Imaging cellular behavior. 10. Imaging cell metabolism. 11. Intravital imaging of cancer cell migration in vivo. 12. Imaging cellular signaling in vivo using fluorescent protein biosensors. 13. Imaging cell adhesion and migration. 14. Imaging the living eye. Section III: Whole-organ and whole-organism imaging. 15. Heart imaging. 16. Visualizing hepatic immunity through the eyes of intravital microscopy. 17. Optical imaging of the mammalian oviduct in vivo. 18. Immune system imaging. 19. Brain imaging in live mice. 20. Live imaging of zebrafish. 21. Whole-body fluorescence imaging in cancer research. 22. Large-scale fluorescence imaging in neuroscience

Margarida Barroso is a Professor at the Department of Molecular and Cellular Physiology, Albany Medical College (Albany, NY). Xavier Intes is a Professor in the Biomedical Engineering Department and Co-Director of the Center for Modeling, Simulation and Imaging for Medicine (CeMSIM),at Rensselaer Polytechnic Institute (Troy, U.S.).

• Section I: Overview of Imaging Methods and Instrumentation. 1. Fluorescence microscopy techniques. 2. Intra-vital microscopy, M Perro. 3. An introduction to live-cell super-resolution imaging. 4. Endoscopic Optical Coherence Tomography: Technologies and Applications. 5. Bioluminescence. 6. Macroscopic Fluorescence Imaging. 7. Optical Coherence Tomography. 8. Multiscale Photoacoustic Imaging. 9. Fluorescence Lifetime: Techniques, Analysis & Applications in the Life Sciences. Section II: Imaging cellular behavior. 10. Imaging cell metabolism. 11. Intravital imaging of cancer cell migration in vivo. 12. Imaging cellular signaling in vivo using fluorescent protein biosensors. 13. Imaging cell adhesion and migration. 14. Imaging the living eye. Section III: Whole-organ and whole-organism imaging. 15. Heart imaging. 16. Visualizing hepatic immunity through the eyes of intravital microscopy. 17. Optical imaging of the mammalian oviduct in vivo. 18. Immune system imaging. 19. Brain imaging in live mice. 20. Live imaging of zebrafish. 21. Whole-body fluorescence imaging in cancer research. 22. Large-scale fluorescence imaging in neuroscience