Introduction to Fluorescence

David M. Jameson is a professor in the Department of Cell and Molecular Biology at the John A. Burns School of Medicine, University of Hawaii at Manoa, having previously served there as professor and chairman of the Department of Biochemistry and Biophysics. He earned his Ph.D. in biochemistry from the University of Illinois at Urbana-Champaign, where his graduate thesis advisor was Professor Gregorio Weber. Prior to his move to the University of Hawaii, he was a postdoctoral fellow at the University of Illinois at Urbana-Champaign and the CNRS, University of Paris-South and he was an assistant professor in the Department of Pharmacology at the University of Texas Southwestern Medical Center. Dr. Jameson is the co-organizer of the International Weber Symposia on Innovative Fluorescence Methodologies in Biochemistry and Medicine, which have been held every three years since 1986 (since 1995 in Hawaii). He serves on the editorial boards of The Scientific World Journal, Analytical Biochemistry, and Methods and Applications in Fluorescence. He is a member of the advisory board for the Laboratory for Fluorescence Dynamics (supported by the National Institutes of Health) at the University of California, Irvine. He was the recipient of the 2004 Gregorio Weber Award for Excellence in Fluorescence Theory/Application.

"An essential contribution to educating scientists in the principles of fluorescence. It will also be an important addition to the libraries of practitioners applying the principles of molecular fluorescence." -Ken Jacobson, Kenan Distinguished Professor of Cell Biology and Physiology, University of North Carolina at Chapel Hill "An exquisite compendium of fluorescence and its applications in biochemistry enriched by a very exciting historical perspective. This book will become a standard text for graduate students and other scientists." -Drs. Zygmunt (Karol) Gryczynski and Ignacy Gryczynski, University of North Texas Health Science Center "... truly a masterwork, combining clarity, precision, and good humor. The reader, novice or expert, will be pleased with the text and will not stop reading. It is a formidable account of the fluorescence field, which has impacted the life sciences so considerably in the last 60 years." -Jerson L. Silva, M.D., Ph.D., Professor and Director, National Institute of Science and Technology for Structural Biology and Bioimaging, Universidade Federal do Rio de Janeiro "Dr. Jameson's book fills a gap in the literature available for the student approaching the applications of fluorescence techniques as well as for the researcher practicing them daily in the laboratory setting. The subject is presented by the author in a clear didactic manner; the anecdotes and historical references make the book truly a delight to read." -Dr. Beniamino Barbieri, President, ISS Inc. "An excellent textbook! This comprehensive overview written at an introductory level covers fundamental aspects, principles of instrumentation, and practical applications of biological fluorescence, while providing many valuable tips. In essence, this book reflects the excellent didactic skills and vast experience of the author in research and teaching fluorescence. Highly recommendable for undergraduate teaching. Also excellent material for active researchers who are learning to apply fluorescence methods." -Prof. Luis Bagatolli, Center for Biomembrane Physics, Department of Biochemistry and Molecular Biology, University of Southern Denmark "I am very impressed by the author's lucid treatment. This book is a 'must read' for students and researchers." -Prof. Amitabha Chattopadhyay, Ph.D., FRSC, J.C. Bose Fellow, Outstanding Scientist, Centre for Cellular & Molecular Biology, Hyderabad

• Introduction. Absorption of Light. Instrumentation. Emission and Excitation Spectra. Polarization and Anisotropy. Time-Resolved Fluorescence. Quantum Yields and Quenching. Forster Resonance Energy Transfer. Brief Overview of Fluorescence Microscopy. Fluorophores. Intrinsic Protein Fluorescence. Appendix. Index.