Genetic Engineering

Jane K. Setlow, PhD. has championed the field of genetics for over 30 years, agreeing to edit Genetic Engineering: Principles & Methods since its inception in 1979. An internationally recognized scientist with numerous publications, Dr. Setlow is the former Chairperson of the NIH Recombinant DNA Molecule Advisory Committee (RAC). Her lab can found at Brookhaven National Laboratory, Upton, New York.

• Genomic Footprinting.- Theoretical and Computer Analysis of Protein Primary Sequences: Structure Comparison and Prediction.- Affinity Chromatography of Sequence-Specific DNA-Binding Proteins.- Applications of the Firefly Luciferase as a Reporter Gene.- Fluorescence-Based Automated DNA Sequence Analysis.- Phosphorothioate-Based Oligonucleotide-Directed Mutagenesis.- Design and Use of Agrobacterium Transformation Vectors.- Cell Commitment and Determination in Plants.- Plasmids Derived from Epstein-Barr Virus: Mechanisms of Plasmid Maintenance and Applications in Molecular Biology.- Chromosome Jumping: A Long Range Cloning Technique.- Isolation of Intact mRNA and Construction of Full-Length cDNA Libraries: Use of a New Vector, ?gt22, and Primer-Adapters for Directional cDNA Cloning.- The Use of Transgenic Animal Techniques for Livestock Improvement.- Plant Reporter Genes: The Gus Gene Fusion System.- Structure of the Genes Encoding Proteins Involved in Blood Clotting.