Single Cell Protein Analysis

Anup Singh Mailstop 9291 Sandia National Laboratories 7011 East Ave Livermore, CA aksingh@sandia.gov

• Single-Cell Western Blotting.- A Microfluidic Device for Immunoassay-Based Protein Analysis of Single E. Coli Bacteria.- Enzyme-Linked ImmunoSpot (ELISpot) for Single Cell Analysis.-     Photocleavable DNA Barcoding Antibodies for Multiplexed Protein Analysis in Single Cells.- Genome-Wide Analysis of Protein and mRNA Copy Numbers in Single Escherichia Coli Cells with Single-Molecule Sensitivity.- Microfluidic Flow Cytometry for Single Cell Protein Analysis.- Microfluidic Image Cytometry for Single Cell Phenotyping of Human Pluripotent Stem Cells.- Characterizing Phenotypes and Signaling Networks of Single Human Cells by Mass Cytometry.- Multiplex Peptide-MHC Tetramer Staining with Mass Cytometry.- Imaging and Mapping of Tissue Constituents at the Single Cell Level Using MALDI MSI and Quantitative Laser Scanning Cytometry.- SPLIFF: A Single Cell Method to Map Protein-Protein Interactions in Time and Space.- Microfluidic Proximity Ligation Assay for Profiling Signaling Networks with Single-Cell Resolution.- Dynamics and Interactions of Individual Proteins in the Membrane of Single, Living Cells.- Microfluidics Enabled Enzyme Activity Measurement in Single Cells.- Microfluidic Chemical Cytometry for Enzyme Assays of Single Cells.- Quantitative Detection of Nucleocytoplasmic Transport of Native Proteins in Single Cells.