G Protein-Coupled Receptor Screening Assays

Methods and Protocols

AvDuarte Miguel F. Prazeres,Sofia Aires M. Martins

Häftad, Engelska, 2016

Del i serien Methods in Molecular Biology

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Beskrivning

This volume explores the considerable efforts that have been directed towards the development of G Protein-Coupled Receptors (GPCR) screening assays in order to disclose GPCR acting compounds, elucidate signaling mechanisms or evaluate compound’s efficacy.

Produktinformation

Utgivningsdatum:2016-09-24
Mått:178 x 254 x 16 mm
Vikt:653 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:296
Förlag:Humana Press Inc.
ISBN:9781493955800

Utforska kategorier

Biokemi inom Naturvetenskap och teknik
Farmakologi inom Medicin

Innehållsförteckning

G-Protein Coupled Receptors: An Overview of Signaling Mechanisms and Screening Assays.- Time–Resolved FRET Strategy to Screen GPCR Ligands Library.- Homogeneous Fluorescence Anisotropy-Based Assay for Characterization of Ligand Binding Dynamics to GPCRs in Budded Baculoviruses: The Case of Cy3B-NDP-a-MSH Binding to MC4 Receptors.- Construction of Recombinant HEK293 Cell Lines for the Expression of the Neurotensin Receptor NTSR1.- cAMP Assay for GPCR Ligand Characterization: Application of BacMam Expression System.- Ca2+ Mobilization Assays in GPCR Drug Discovery.- Using Constitutive Activity to Define Appropriate High Throughput Screening Assays for Orphan G Protein-Coupled Receptors.- Monitoring G-Protein Coupled Receptor Activation Using the Protein Fragment Complementation Technique Split TEV.- Quantifying GPCR Internalization: A Focus on the Kisspeptin Receptor.- GPCR Oligomerization Analysis by Means of BRET and dFRAP.- Use of ImageJ to Recover Information from Individual Cells in a G-Protein Coupled Receptor Assay.- Methods to Immobilize GPCR on the Surface of SPR Sensors.- Olfactory Receptor Screening Assay Using Nanovesicle-Immobilized Carbon Nanotube Transistor.- Label-Free Biosensor Assays in GPCR Screening.- Multi-Dimensional GPCR Profiling and Screening Using Impedance-Based Label-Free and Real-Time Assay.- Label-Free Functional Selectivity Assays.- Measurement of Surface-Mediated Ca++ Transients on the Single-Cell Level in a Microfluidic Lab-on-a-chip Environment.- Cell-Based Assays and Animal Models for GPCR Drug Screening.- Computer-Aided Design of GPCR Ligands.