MicroRNAs in Development

From the reviews:“Well edited book by Prof. Tamas Dalmay provides a complete (as for today !) overview of the role played by microRNAs … . Thanks to this book I think that a multitude of readers can better understand how the genome express itself in time and space. … gives to the scientific community a clear vision of a five dimensional world in which to investigate: the three anatomical spaces (x, y and z) plus the heterochronic and heterotopic dimensions of gene expressions thanks to the microRNAs.” (Manuela Monti, European Journal of Histochemistry, July, 2011)

• In situ Detection of microRNAs in Animals.- Detection of microRNAs in Plants by In situ Hybridization.- Detecting Animal and Plant microRNAs by Northern Blotting.- Profiling microRNAs by Real-time PCR.- Detection of Small RNAs and microRNAs using Deep Sequencing Technology.- Detection of microRNAs in Prostate Cancer Cells by microRNA Array.- microRNA Knock Down by Cholesterol Conjugated Antisense Oligos in Mouse Organ Culture.- Protocols for Use of Homologous Recombination Gene-targeting to Produce microRNA Mutants in Drosophila.- Engineering Elements for Gene Silencing: The Artificial microRNAs Technology.- Mimicry Technology: Suppressing Small RNA Activity in Plants.- Experimental Validation of MicroRNA Targets using a Luciferase Reporter System.- Experimental Identification of microRNA Targets by Immunoprecipitation of Argonaute Protein Complexes.- Comprehensive Identification of miRNA Target Sites in Live Animals.- Target Validation of Plant microRNAs.- A High-throughput Sequencing-based Methodology to Identify All Uncapped and Cleaved RNA Molecules in Eukaryotic Genomes