Lipases and Phospholipases

Methods and Protocols

AvGeorgina Sandoval

Häftad, Engelska, 2016

Del i serien Methods in Molecular Biology

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Beskrivning

Lipases are the most applied enzymes in organic synthesis due to their broad substrate acceptance and because of the availability of the molecular, biochemical, themodynamical and solvent engineering tools, which allows the optimization of lipases and lipase-catalyzed reactions.

Produktinformation

Utgivningsdatum:2016-08-23
Mått:178 x 254 x 31 mm
Vikt:1 047 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:547
Förlag:Humana Press Inc.
ISBN:9781493958771

Utforska kategorier

Biokemi inom Naturvetenskap och teknik
Biologi inom Naturvetenskap och teknik

Innehållsförteckning

Lipases: an Overview.-Lipases or Esterases: Does it Really Matter? Toward a New Bio-Physico-Chemical Classification.-Study of New Feruloyl Esterases to Understand Lipase Evolution: The Case of Bacillus Flexus.-Phospholipases: an Overview.-High-Throughput Screening Method for Lipases/Esterases.-Functional-Based Screening Methods for Lipases, Esterases and Phospholipases in Metagenomic Libraries.-Plant Lipases:Partial Purification of C. papaya Lipase.-Plant Phospholipases: an Overview.-Lipase and Phospholipase Inhibitors: Design and Applications.-High-Throughput Assay of Secreted Phospholipases A2 Inhibitors.-Heterologous Expression Systems for Lipases: A Review.- Streptomyces phospholipase D Cloning and Production.-Cloning of PLD2 from Baculovirus for Studies in Inflammatory Responses.-Lipases Production by Solid-State Fermentation: The Case of Rhizopus Homothallicus in Perlite.-Lipases and Esterases from Extremophiles: Overview and Case Example of the Production and Purification of an Esterase from Thermus thermophilus HB27.-Purification, Biochemical and Kinetic Properties of Recombinant Staphylococcus aureus Lipase.-Phospholipase A2 Purification and Characterization; A Case Study.-The Yeast Yarrowia lipolytica as a Generic Tool for Molecular Evolution of Lipases.-Molecular Modeling of Lipase Binding to a Substrate-Water Interface.-Different Strategies for Hyperactivation of Lipase Biocatalysts.-Rational Design ofImmobilized Lipases and Phospholipases.-Thermodynamical Methods for the Optimization of Lipase Catalyzed Reactions.-Lipases as Biocatalysts for the Synthesis of Structured Lipids.-Lipase-Catalyzed Modification of Phenolic Antioxidants.-Lipases as Catalysts in Synthesis of Fine Chemicals.-Lipases in Green Chemistry: Acylation and Alcoholysis on Steroids and Nucleosides.-Lipases as Biocatalyst for Biodiesel Production.-Biopolymer Synthesis Catalyzed by Lipases.-Phospholipases in Food Industry – A Review.-Lipase and Phospholipase Biosensors: a Review.