Plant Salt Tolerance

Methods and Protocols

AvSergey Shabala,Tracey Ann Cuin

Häftad, Engelska, 2016

Del i serien Methods in Molecular Biology

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Beskrivning

Plant Salt Tolerance: Methods and Protocols describes recent advances and techniques employed by researchers to understand the molecular and ionic basis of salinity tolerance and to investigate the mechanisms of salt stress perception and signalling in plants.

Produktinformation

Utgivningsdatum:2016-08-23
Mått:178 x 254 x 25 mm
Vikt:837 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:432
Förlag:Humana Press Inc.
ISBN:9781493959396

Utforska kategorier

Växtböcker inom Djur och Natur
Botanik inom Naturvetenskap och teknik
Biovetenskap inom Naturvetenskap och teknik

Innehållsförteckning

Patch-Clamp Protocols to Study Cell Ionic Homeostasis under Saline Conditions.- Studying Plant Salt Tolerance with the Voltage Clamp Technique.- Using the Multifunctional Xylem Pprobe for In situ Studies of Plant Water and Ion Relations under Saline Conditions.- Measuring Intracellular Ion Concentrations with Multi-Barrelled Microelectrodes.- Single-Cell Sampling and Analysis (SiCSA).- Non-Invasive Flux Measurements Using Microsensors: Theory, Limitations and Systems.- Quantifying Kinetics of Net Ion Fluxes from Plant Tissues by Non-Invasive Microelectrode Measuring MIFE Technique.- Quantitative Cryo-Analytical Scanning Electron Microscopy (CEDX): An Important Technique Useful for Cell-Specific Localization of Salt.- Fluorescence Lifetime Imaging (FLIM) Measurements in Salinity Research.- Cytosolic Ca2+ Determinations in Studying Plant Responses to Salinity and Oxidative Stress.- Infrared Thermography in Plant Phenotyping for Salinity Tolerance.- In vivo Imaging of Nitric Oxide and Reactive Oxygen Species Using Laser Scanning Confocal Microscopy.- Metabolomics for Salinity Research.-Purification of Plant Plasma Membranes by Two-Phase Partitioning and Measurement of H+ Pumping.-Determination of Reactive Oxygen Species in Salt-Stressed Plant Tissues.-Quantification of the Antioxidant Activity in Salt-Stressed Tissues.-Quantification of Abscisic Acid, Cytokinin and Auxin Content in Salt-Stressed Plant Tissues.-Fluorescence-Activated Cell Sorting for Analysis of Cell Type-Sspecific Responses to Salinity Stress in Arabidopsis and Rice.-Transformation Using Controlled cDNA Overexpression System.-Transcriptome Analysis of Membrane Transporters in Response to Salinity Stress.-Marker-Assisted Selection in Plant Breeding for Salinity Tolerance.-Transcriptomics on Small Samples.-Plastid Transformation for Abiotic Stress Tolerance in Plants.-Manipulating Expression of Tonoplast Transporters.-Using Heterologous Expression Systems to Characterize Potassium and Sodium Transport Activities.-Isotope Techniques to Study Kinetics of Na+ and K+ Transport Under Salinity Conditions.-Trait Dissection of Salinity Tolerance with Plant Phenomics.-Measuring Soil Salinity.