Phytoplasma

Methods and Protocols

AvMatt Dickinson,Jennifer Hodgetts

Häftad, Engelska, 2016

Del i serien Methods in Molecular Biology

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Beskrivning

Phytoplasmas are a group of bacteria that are capable of multiplying and causing severe diseases in a wide range of plant species and are transported between plants by insect vectors in which the bacteria can also multiply.

Produktinformation

Utgivningsdatum:2016-08-23
Mått:178 x 254 x 24 mm
Vikt:815 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:421
Förlag:Humana Press Inc.
ISBN:9781493959655

Utforska kategorier

Växtböcker inom Djur och Natur
Botanik inom Naturvetenskap och teknik
Biovetenskap inom Naturvetenskap och teknik

Innehållsförteckning

The Phytoplasmas - An Introduction.- Techniques for the Maintenance and Propagation of Phytoplasmas in Glasshouse Collections of Catharanthus roseus.- Micropropagation and Maintenance of Phytoplasmas in Tissue Culture.- Dodder Transmission of Phytoplasmas.- Insect Maintenance and Transmission.- Capturing Insect Vectors of Phytoplasmas.- Insect Vector Transmission Assays.- Molecular Identification of Phytoplasma Vector Species.- Dienes’ Staining and Light Microscopy for Phytoplasma Visualization.- DAPI Staining and Fluorescence Microscopy Techniques for Phytoplasmas.- Visualisation of Phytoplasmas using Electron Microscopy.- Automated DNA Extraction for Large Numbers of Plant Samples.- DNA Extraction from Arborescent Monocots and How To Deal With Other Challenging Hosts.- Nested PCR and RFLP Analysis Based on the 16S rRNA Gene.- PCR and RFLP Analyses Based on the Ribosomal Protein Operon.- Tuf and secY PCR Amplification and Genotyping of Phytoplasmas.- PCR Analysis of Phytoplasmas Based on the secA Gene.- Single-Strand Conformation Polymorphism Analysis for Differentiating Phytoplasma Strains.- Microarrays for Universal Detection and Identification of Phytoplasmas.- T-RFLP for Detection and Identification of Phytoplasmas in Plants.- Real-Time PCR for Universal Phytoplasma Detection and Quantification.- A Real-Time PCR Detection System for the Bois Noir and Flavescence Dorée Phytoplasmas and Quantification of the Target DNA.- Real-Time PCR for Specific Detection of Three Phytoplasmas from the Apple Proliferation Group.- Reverse Transcription-PCR for Phytoplasma Detection Utilizing Crude Sap Extractions.- In-Field Diagnostics Using Loop-Mediated Isothermal Amplification (LAMP).- DNA Bar-Coding for Phytoplasma Identification.- Phylogenetic Analyses of Phytoplasmas Based on Whole-Genome Comparison.- The iPhyClassifier, An Interactive Online Tool for Phytoplasma Classification and Taxonomic Assignment.- Phytoplasma Proteomic Analysis.- Preparation ofPhytoplasma Membrane Recombinant Proteins.- Phytoplasma Plasmid DNA Extraction.- Caesium Chloride-Bisbenzimide Gradients for Separation of Phytoplasma and Plant DNA.- Pulsed-Field Gel Elecrophoresis for Isolation of Full-Length Phytoplasma Chromosomes From Plants.- Mapping the Phytoplasma Chromosome.