PCR Detection of Microbial Pathogens

Häftad, Engelska, 2016

Del i serien Methods in Molecular Biology

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Beskrivning

PCR methods for the detection of microbial pathogens have made relatively little impact in diagnostic microbiology laboratories due to the common decision to use expensive commercially produced tests rather than the cheaper alternative of developing one’s own tests or introducing tests developed by other workers.

Produktinformation

Utgivningsdatum:2016-08-23
Mått:178 x 254 x 19 mm
Vikt:626 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:317
Upplaga:2
Förlag:Humana Press Inc.
ISBN:9781493960996

Utforska kategorier

Biovetenskap inom Naturvetenskap och teknik
Biologi inom Naturvetenskap och teknik

Innehållsförteckning

Real-time Quantitative PCR, Pathogen Detection and MIQE.- Overcoming Inhibition in Real-time Diagnostic PCR.- Quality in the Molecular Microbiology Laboratory.- Pre-Analytical Sample Treatment and DNA Extraction Protocols for the Detection of Bacterial Pathogens from Whole Blood.- Detection of Bacterial Contamination in Platelet Concentrates using Flow Cytometry and Real-time PCR Methods.- Multiplex Real-time PCR Assay for the Detection of Meticillin-Resistant Staphylococcus aureus and Panton-Valentine Leukocidin from Clinical Samples.- PCR Detection of Haemophilus influenzae from Respiratory Specimens.- Rapid Detection of Atypical Respiratory Bacterial Pathogens by Real-time PCR.- Use of Multiple Real-time PCR Assays for the Diagnosis of Bodetella spp in Clinical Specimens.- Detection of Mycoplasma pneumoniae by Real-time PCR.- Real-Time PCR Assay for the Diagnosis of Pneumocystis jirovecii Pneumonia.- Rapid Identification of Mycobacteria and Rapid Detection of Drug Resistance in Mycobacterium tuberculosis in Cultured Isolates and in Respiratory Specimens.- Direct Detection of Mycobaterium ulcerans in Clinical Specimens and Environmental Samples.- Detection of Bartonella spp. DNA in Clinical Specimens using an Internally Controlled Real-time PCR Assay.- Direct Identification of Multiple Genital Pathogens in Urine using Multiplex PCR-based Reverse Line Blot Assays.- Diagnosis of Clostridium difficile Infection using Real-time PCR.- Detection of Pathogenic Leptospira spp. through Real-time PCR (qPCR) Targeting the LipL32 Gene.- Sensitive and Rapid Detection of Campylobacter jejuni and Campylobacter coli using Loop-mediated Isothermal Amplification.- PCR Detection of Helicobacter pylori in Clinical Samples.- Rapid Detection of the Escherichia coli Genospecies in Water by Conventional and Real-time PCR.- Multiplex Real Time PCR (MRT-PCR) for Diarrheagenic Escherichia coli.