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Beskrivning
New pathways have been discovered and expanded our knowledge of the ways in which genes and gene products affect bone cells and thereby bone mass and bone strength.
From the reviews of the second edition:“This volume presents detailed modern experimental methods to study bone biology. For bone researchers.” (Pediatric Endocrinology Reviews (PER), Vol. 9 (3), March, 2012)
Innehållsförteckning
Primary Human Osteoblast Cultures.- Osteoblast Isolation from Murine Calvaria and Long Bones.- Rat Osteoblast Cultures.- Isolation of primary avian Osteocytes.- Isolation of Mouse Osteocytes using Cell Fractionation for Gene Expression Analysis.- Studying Osteocyte Function using the Cell Lines MLO-Y4 and MLO-A5.- Isolation, Differentiation and Characterisation of Human Bone Marrow Stem Cells in vitro and in vivo.- Rodent Osteoclast Cultures.- Isolation and Culture of Primary Chicken Osteoclasts.- ISOLATION AND PURIFICATION OF RABBIT OSTEOCLASTS.- Generation of Human Osteoclasts from Peripheral Blood.- Osteoclast Formation in Mouse Co-Cultures.- Rankl- Mediated Osteoclast Formation from Murine RAW 264.7 Cells .- Transfection of Osteoclasts and Osteoclast Precursors.- Analysis of Signalling Pathways by Western Blotting and Immunoprecipitation.- Analysis of Transcriptional Regulation in Bone Cells.- Extraction of Nucleic Acids from Bone.- Analysis of Gene Expression in Bone by Quantitative RT/PCR .- Histomorphometry in Rodents.- Studying Gene Expression in Bone by in situ Hybridization.- Immunostaining of Skeletal Tissue.- Techniques for the Study of Apoptosis in Bone.- Transmission Electron Microscopy of Bone.- Scanning Electron Microscopy of Bone.- Fluorescence Imaging of Osteoclasts using Confocal Microscopy.- Live Imaging of Bone Cell and Organ Cultures.- Analysis of Bone Architecture in Rodents using Micro Computed Tomography.- Bone Measurements by Peripheral Quantitative Computed Tomography in Rodents.- Quantitative X-ray Imaging of Rodent Bone by Faxitron.- Bioluminescence Imaging of Bone Metastasis in Rodent.- Fourier Transform Infrared Imaging of Bone.- Raman Microscopy of Bone.- The Calvarial Injection Assay.- Ovariectomy/Orchidectomy in Rodents.- Mechanical Properties of Bone ex vivo.- Mechanical Stimulation of Bone Cells using Fluid Flow.- using CELL and organ culture models TO analyse responses OF bone cellS to mechanical STIMULATION .In vivo Mechanical Loading.
