Mammalian Cell Viability

Methods and Protocols

AvMartin J. Stoddart

Häftad, Engelska, 2016

Del i serien Methods in Molecular Biology

1 283 kr

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Beskrivning

Whether the question is one of basic cell survival, or whether it is being used to correlate cell number to some other factor such as matrix synthesis, an estimate of cell viability is universally required.

Produktinformation

Utgivningsdatum:2016-08-23
Mått:178 x 254 x 14 mm
Vikt:482 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:240
Förlag:Humana Press Inc.
ISBN:9781493961535

Utforska kategorier

Innehållsförteckning

Cell Viability Assays: Introduction.- Cell Viability Analysis Using Trypan Blue: Manual and Automated Methods.- Estimation of Cell Number Based on Metabolic Activity: The MTT Reduction Assay.- WST8 Analysis of Cell Viability During Osteogenesis of Human Mesenchymal Stem Cells.- Assessment of Cell Proliferation with Resazurin-Based Fluorescent Dye.- The xCELLigence System for Real-Time and Label-Free Monitoring of Cell Viability.- Analysis of Tumor and Endothelial Cell Viability and Survival Using Sulforhodamine B (SRB) and Clonogenic Assays.- Annexin V / 7-AAD Staining in Keratinocytes.- Measurement of Caspase Activity: From Cell Populations to Individual Cells.- Rapid Quantification of Cell Viability and Apoptosis in B-Cell Lymphoma Cultures Using Cyanine SYTO Probes.- Multiplexing Cell Viability Assays.- Cytotoxicity Testing: Measuring Viable Cells, Dead Cells, and Detecting Mechanism of Cell Death.- Fluorescein Diacetate for Determination of Cell Viability in 3D Fibroblast-Collagen-GAG Constructs.- Confocal Imaging Protocols for Live/Dead Staining in 3-Dimensional Carriers.- Viability Assessment of Osteocytes Using Histological Lactate Dehydrogenase Activity Staining on Human Cancellous Bone Sections.- Measuring Glutamate Receptor Activation-Induced Apoptotic Cell Death in Ischemic Rat Retina Using the TUNEL Assay.- Exploiting the Liberation of Zn2+ to Measure Cell Viability.- Non-Invasive Bioluminescent Quantification of Viable Stem Cells in Engineered Constructs.- Raman Micro-Spectroscopy as a Non-Invasive Cell Viability Test.- Closed Ampoule Isothermal Microcalorimetry (IMC) for Continuous Real-Time Detection and Evaluation of Cultured Mammalian Cell Activity and Responses.-Digital Image Processing of Live/Dead Staining.