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Beskrivning
Despite the many milestones in cystic fibrosis (CF) research, progress toward curing the disease has been slow, and it is increasingly difficult to grasp and use the already wide and still growing range of diverse methods currently employed to study CF so as to understand it in its multidisciplinary nature.
Introduction to Part I: Overview of Approaches to Study Cystic Fibrosis Pathophysiology.- Imaging CFTR Protein Localization in Cultured Cells and Tissues.- CFTR Regulation of Epithelial Sodium Channel.- Methods for Evaluating Inflammation in Cystic Fibrosis.- Methods for ASL Measurements and Mucus Transport Rates in Cell Cultures.- Measurement of Fluid Secretion from Intact Airway Submucosal Glands.- Measurements of Intracellular Calcium Signals in Polarized Primary Cultures of Normal and Cystic Fibrosis Human Airway Epithelia.- Identification and Quantification of Mucin Expression.- Methods to Classify Bacterial Pathogens in Cystic Fibrosis.- Approaches to Study Differentiation and Repair of Human Airway Epithelial Cells.- Introduction to Part II: Omics in the Biology of Cystic Fibrosis.- Microarray mRNA Expression Profiling to Study Cystic Fibrosis.- Quantitative Differential Proteomics of Cystic Fibrosis Cell Models by SILAC (Stable Isotope Labelling in Cell Culture).- Application of Mass Spectrometry to Study Proteomics and Interactomics in Cystic Fibrosis.- Functional Genomics Assays to Study CFTR Traffic and ENaC Function.- New Lipidomic Approaches in Cystic Fibrosis.- Introduction to Part III: Resources for CFTR Research.- Primary Epithelial Cell Models for Cystic Fibrosis Research.- Comparative Biology of Cystic Fibrosis Animal Models.- CFTR Folding Consortium: Methods Available for Studies of CFTR Folding and Correction.- Evaluation of the Disease Liability of CFTR Variants.
