Epitope Mapping Protocols

Inbunden, Engelska, 2018

Del i serien Methods in Molecular Biology

1 877 kr

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Beskrivning

The chapters in this book are divided into four parts: Part One looks at the entire native antigen and covers traditional structural biology techniques such as nuclear magnetic resonance and x-ray crystallography.

Produktinformation

Utgivningsdatum:2018-05-01
Mått:178 x 254 x 21 mm
Vikt:825 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:284
Upplaga:3
Förlag:Humana Press Inc.
ISBN:9781493978397

Utforska kategorier

Biokemi inom Naturvetenskap och teknik
Onkologi inom Medicin

Innehållsförteckning

An Introduction to Epitope Mapping.- Epitope Mapping of Antibody-Antigen Interactions with X-Ray Crystallography.- Mapping Antibody Epitopes by Solution NMR Spectroscopy: Practical Considerations.- Label-Free Cell-Based Assay for Characterization of Biomolecules and Receptors.- Probing Antibody Binding Sites on G Protein-Coupled Receptors Using Genetically-Encoded Photo-Activatable Cross-Linkers.- Rapid Mapping of Protein Binding Sites and Conformational Epitopes by Coupling Yeast Surface Display to Chemical Labeling and Deep Sequencing.- Epitope Mapping Using Yeast Display and Next Generation Sequencing.- B-Cell Epitope Mapping Using a Library of Overlapping Synthetic Peptides in an Enzyme-Linked Immunosorbent Assay.- Array-In-Well Epitope Mapping of Phage-Displayed Antibodies.- Epitope Mapping of Antibodies Using Bacterial Cell Surface Display of Gene Fragment Libraries.- Discontinuous Epitope Mapping of Antibodies Using Bacterial Cell Surface Display of Folded Domains.- Kinetic Analysis and Epitope Binning Using Surface Plasmon Resonance.- Epitope Binning of Human Monoclonal Antibodies in Classical Sandwich and In-Tandem Orientation Using the Octet System Based on Biolayer Interferometry.- Antibody Specificity Profiling Using Protein Microarrays.- High-Density Antigen Microarrays for Assessment of Antibody Selectivity and Off-Target Binding.- Multiplexed Antigen Bead Arrays for Assessment of Antibody Selectivity and Epitope Mapping.- Antibody Biomarker Discovery Via In Vitro Evolution of Peptides.- Bioinformatics Tools for the Prediction of T Cell Epitopes.