This second edition expands on the first edition with new chapters describing methods for studyingcell movement, molecular components involved in chemotaxis, spatiotemporaldynamics of signaling components, and quantitative modeling, as well as severalupdated chapters from the first edition. Various methods to investigatedirectional cell growth and movements are presented in Chapters 1-20. Thesechapters contains experimental procedures to visualize and measure migrationbehaviors of different kinds of organisms, including chemotropism in thebudding yeast; cell growth and migration of D. discoideum; border cellmigration in Drosophila; chemotaxis of mouse and human neutrophils; andHIV-induced T cell chemotactic response. Chemotaxis: Methods and Protocols,Second Edition also contains microscopy procedures for studying breast cancercell migration, tumor cell invasion in vivo, and axon guidance. The book concludeswith Chapters 21-27 describing methods that measure spatiotemporal dynamics ofsignaling components involved in chemotaxis; introduce imaging techniques, suchas TRIF, BRET, FRET, and single-molecule microscopy; and mathematical models ofexperimentally generated chemoattractant gradients. Written in the highlysuccessful Methods in Molecular Biology series format, chapters includeintroductions to their respective topics, lists of the necessary materials andreagents, step-by-step, readily reproducible laboratory protocols, and tips ontroubleshooting and avoiding known pitfalls.Cutting edge and thorough,Chemotaxis: Methods and Protocols, Second Edition is a valuable resource foranyone who is interested in the diverse methodologies that are propellingchemotaxis research forward.
