Chemotaxis

Methods and Protocols

AvTian Jin,Dale Hereld

Häftad, Engelska, 2018

Del i serien Methods in Molecular Biology

1 170 kr

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Beskrivning

This second edition expands on the first edition with new chapters describing methods for studyingcell movement, molecular components involved in chemotaxis, spatiotemporaldynamics of signaling components, and quantitative modeling, as well as severalupdated chapters from the first edition.

Produktinformation

Utgivningsdatum:2018-05-26
Mått:178 x 254 x 24 mm
Vikt:822 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:425
Upplaga:2
Förlag:Humana Press Inc.
ISBN:9781493980598

Utforska kategorier

Cellbiologi inom Naturvetenskap och teknik
Biologi inom Naturvetenskap och teknik

Innehållsförteckning

In Situ Assays of Chemotropism duringYeast Mating.- ImagingPolarization in Budding Yeast Migration of Dictyostelium discoideum to theChemoattractant Folic Acid.- MitochondrialStress Tests Using Seahorse Respirometry on Intact Dictyostelium discoideum Cells.- StudyingChemoattractant Signal Transduction Dynamics in Dictyostelium by BRET.- Wave Patternsin Cell Membrane and Actin Cortex Uncoupled from Chemotactic Signals.- ChemotacticBlebbing in Dictyostelium Cells.- DissectingSpatial and Temporal Sensing in DictyosteliumChemotaxis Using a Wave Gradient Generator.- Employing Dictyostelium as an Advantageous 3RsModel for Pharmacogenetic Research.- Identificationof Associated Proteins by Immunoprecipitation and Mass Spectrometry Analysis.- BiochemicalResponses to Chemically Distinct Chemoattractants during the Growth andDevelopment of Dictyostelium.- Live Imaging ofBorder Cell Migrationin Drosophila.- ShRNA-InducedGene Knock-Down In Vivo to Investigate Neutrophil Function.- StudyingNeutrophil Migration In Vivo Using Adoptive Cell Transfer.- IntravitalTwo-Photon Imaging of Lymphocytes Crossing High Endothelial Venules andCortical Lymphatics in the Inguinal Lymph Node.- FlowCytometry-Based Quantification of HIV-Induced T Cell Chemotactic Response.- VisualizingCancer Cell Chemotaxis and Invasion in 2D and 3D.- 4D TumorigenesisModel for Quantitating Coalescence, Directed Cell Motility and Chemotaxis,Identifying Unique Cell Behaviors and Testing Anti-Cancer Drugs.- An ExperimentalModel for Simultaneous Study of Migration of Cell Fragments, Single Cells andCell Sheets.- Axon GuidanceStudies Using a Microfluidics-Based Chemotropic Gradient Generator.- Visualization ofActin Assembly and Filament Turnover by In Vitro Multi-Color TIRF Microscopy.- QuantitativeMonitoring Spatiotemporal Activation of Ras and PKD1 Using Confocal FluorescentMicroscopy.- FluorescenceReadout of a Patch Clamped Membrane by Laser Scanning Microscopy.- Use of ResonanceEnergy Transfer Techniques for In Vivo Detection of Chemokine ReceptorOligomerization.- Multi-StateTransition Kinetics of Intracellular Signaling Molecules by Single-MoleculeImaging Analysis.- Mathematics ofExperimentally Generated Chemoattractant Gradients.- ModelingExcitable Dynamics of Chemotactic Networks.